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HTH17MAG-14K MILLIPLEX MAP Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology
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HTH17MAG-14K
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
GM-CSF IFN-γ IL-1β IL-2 IL-4 IL-5 IL-6 IL-9 IL-10 IL-12 (p70) IL-13 IL-15 IL-17A IL-17F IL-17E/IL-25 IL-21 IL-22 IL-23 IL-27 IL-28A IL-31 IL-33 MIP-3α/CCL20 TNF-α TNFβ HMplexLuminex xMAP
Description
Catalogue NumberHTH17MAG-14K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay
OverviewEMD Millipore’s MILLIPLEX® MAP Human TH17 Magnetic Bead Panel is a 25-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α and TNFβ.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel


MILLIPLEX® MAP offers you:
o The ability to choose any combination of analytes from our panel of seven analytes to design a custom kit that better meets your needs.
o A convenient “all-in-one” box format that gives you the assurance that you will have all the necessary reagents you need to run your assay.

EMD Millipore’s MILLIPLEX® MAP Human TH17 Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background InformationCD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFNγ, secrete IFNγ that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation.

Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn's disease and autoimmune uveitis. In addition Th17 cells play an important role in tumor development, progression and metastasis, potentially in both promoting and inhibiting tumor growth.

The MILLIPLEX® MAP Human TH17 Magnetic Bead Panell enables you to focus on the relevance and therapeutic potential of the modulation of CD4 T-helper cell cytokines in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-assay:< 10
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Th17 panel analytes in serum, plasma or tissue/cell lysate and culture supernatant
Biological Information
Species Reactivity
  • Human
Analytes Available
  • GM-CSF
  • IFN-γ
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-9
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-15
  • IL-17A
  • IL-17F
  • IL-17E/IL-25
  • IL-21
  • IL-22
  • IL-23
  • IL-27
  • IL-28A
  • IL-31
  • IL-33
  • MIP-3α/CCL20
  • TNF-α
  • TNFβ
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 79 - 107%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range
  • IL-17 & IL-4, 24 - 100,000 pg/mL
  • GM-CSF & IL-27, 61 - 250,000 pg/mL
  • IFNγ, 10 - 40,000 pg/mL
  • IL-10, 1 - 5000 pg/mL
  • MIP-3α, IL-1β, IL-12 (p70), IL-15, IL-21 & IL-33, 5 - 20,000 pg/mL
  • IL-13, 7 - 30,000 pg/mL
  • IL-17A & IL-2, 12 - 50,000 pg/mL
  • IL-22 & TNFβ, 37 - 150,000 pg/mL
  • IL-9, 9 - 35,000 pg/mL
  • IL-23, 336 - 1,500,000 pg/mL
  • IL-5, 6 - 25,000 pg/mL
  • IL-6 & TNFα, 3 - 10,000 pg/mL
  • IL-17E/IL-25, 488 - 2,000,000
  • IL-28a & IL-31, 49 - 200,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
HTH17MAG-14K 04053252987670

Documentation

Protocols

Title
Protocol- HTH17MAG-14K, HT17MG-14K-PX2, HT17MAG14PMX25BK

MILLIPLEX MAP Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
MAGPIX®and Luminex 200™ - Multiplex Detection of Inflammation Pathway Analytes

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HTH17MAG-14K xPonent Protocol Template Version 3.1
HTH17MAG-14K xPonent Protocol Template Version Magpix
Quality Control Range Sheet- TH17-113 and TH17-213
Quality Control Range Sheet- TH17-114 and TH17-214
Quality Control Ranges: Human Th17 Magnetic Bead Panel

Posters

Title
MILLIPLEX MAP: The Immunology of Cancer
MILLIPLEX®MAP Cell Signaling Wall Chart

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Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits