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Precise and Accurate Counts and Viability Measurements Across Multiple Cell Lines Using the Muse® Cell Count & Viability Assay
In this application note, we show that the Muse® Cell Count & Viability Assay provides superior performance to conventional viability and count measurement by Trypan blue exclusion.
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The assessment of cell concentration in combination with viability is an important step in the characterization of cell health. Cell viability information can be used for monitoring proliferation rates, optimizing growth conditions and normalizing cell data for further studies and assessing the impacts of cytotoxic compounds. Current methods rely on multiple or complex methods to provide these answers, limiting researchers’ ability to simply obtain comprehensive cell health information. Traditional methods may provide inconsistent results, because they are dependent upon single-uptake dyes, which do not discriminate between the various states of cellular demise. There is therefore a crucial need for analytical methods that provide rapid, robust and reproducible count and viability data to enable the efficient, daily execution of cellular research. The Muse® Count and Viability Assay is a simple, rapid, linear, assay that provides cell concentration and viability information and is performed on the Muse® Cell Analyzer. The assay provides superior sensitivity and reproducibility when compared with methods that depend on Trypan blue exclusion.
