Separation of Aminoglycoside Antibiotics on SeQuant® ZIC®-cHILIC


Column SeQuant® ZIC®-cHILIC 100x2.1 mm
(1.50657.0001)		 Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-SeQuant ZIC-Paramomycin-480x388-03052014.jpg
Paromomycin (PAR) 
Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-SeQuant ZIC-Neomycin-480x446-03052014.jpg
Neomycin (NEO)
Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-SeQuant ZIC-Separation of Aminoglycosides-480x315-03052014.jpg
Temperature 50°C
Flow rate 5 µL
Mobile Phase A: acetonitrile with 1% (w/w) formic acid
B: 100 mM ammonium acetate with 3% (w/w) formic acid
Gradient 50 to 95% B
95% B
95 to 50% B		 0-7 min
7-8 min
8-16 min (equilibration)
Inj. volume 5 μL
Sample ptomycin (STR) 5 µg/mL
gentamycin (GEN) 25 µg/mL
paromomycin (PAR),
tobramycin (TOB),
and neomycin (NEO),
all diluted in 30:70 (v/v) acetonitrile / Milli-Q water
Detection Shimadzu LCMS 2012EV, detector voltage: 2.0 kV, heat block and CDL temp: 250 °C, SIM in positive mode: m/z 582 (STR), 464 (GEN), 616 (PAR), 46^8 (TOB), and 615 (NEO)