Separation of Aminoglycoside Antibiotics on SeQuant® ZIC®-cHILIC

Isocratic separations on SeQuant® ZIC®-cHILIC 100x2.1 mm (Ord. No. 1.50657.0001) operated at 23°C with a flow rate of 0.4 mL/min. Eluent was acetonitrile / 100 mM aqueous ammonium acetate pH 4.5, with acetonitrile ratios of 60%, 65%, 70% and 75%, respectively (top to bottom). Injection of 5 µL of adenosine, AMP, ADP, ATP diluted in eluent. UV detection at 254 nm.