Comparison of the Particle Size Distribution of TLC and HPTLC Plates

	HPTLC	TLC
Mean particle size	5-6 μm	10 - 12 μm
Particle size distribution	4 - 8 μm	5 - 20 μm
Layer thickness	200 μm (100 μm)	250 μm (200 μm)
Typical plate height	12 μm	30 μm
Typical migration distance	3 - 6 cm	10 - 15 cm
Typical separation time	3 - 20 min	20 - 200 min
Number of samples per plate	< 36 (72)	< 10
Sample volume	0.1 - 0.5 μl	1 - 5 μl
Detection limits UV absorption	100 - 500 pg	1 - 5 ng
Detection limits fluorescence	5 - 10 pg	50 - 100 pg