EMD Millipore Launches Kit for Analyzing NFкB Translocation by Imaging Flow Cytometry

• Permits quantitative, statistically robust assessment of NFкB translocation
• Allows for analysis at single-cell level
• Optimized and designed for Amnis® imaging flow cytometers

November 19, 2014, Billerica, MA — EMD Millipore, the Life Science division of Merck KGaA, Darmstadt, Germany, today introduced the Amnis® NFкB Translocation kit, which allows researchers to better study the nuclear translocation of NFкB, a transcription factor that plays a central role in regulating key mammalian cell processes, including proliferation, inflammation, immune and stress responses. The new kit uses imaging flow cytometry to obtain statistically significant quantitative assessment of NFкB translocation, as well as visual identification of the translocation at a single-cell level.

The optimized kit, which works with cultured cell lines and whole blood cells, conveniently contains directly-conjugated anti-Human NFкB monoclonal antibody, 7-AAD dye and required buffers. Using the dedicated Nuclear Localization Wizard in the IDEAS software, NFкB translocation can then be studied and quantified in an objective, statistically robust manner. The kit is designed for use with the Amnis® ImageStream®X Mark II and the Amnis® FlowSight® imaging flow cytometers, systems which combine the quantitative power of flow cytometry with the spatial information provided by microscopy.

“With a central role in many cellular responses and human diseases, NFкB continues to be an attractive target for drug discovery,” said Patrick Schneider, Ph.D., Head of Bioscience. “Availability of the Amnis® imaging flow systems in combination with the new NFкB Translocation kit provides immunology and cell biology researchers an easier, higher throughput way to further explore the mechanisms of action of this important transcription factor and reveal insights into targeting its signaling pathway for therapeutic purposes.”

For more information, please visit www.emdmillipore.com/amnis.

About EMD Millipore
EMD Millipore is the Life Science division of Merck KGaA, Darmstadt, Germany and offers a broad range of innovative, performance products, services and business relationships that enable our customers' success in research, development and production of biotech and pharmaceutical drug therapies. Through dedicated collaboration on new scientific and engineering insights, and as one of the top three R&D investors in the Life Science Tools industry, EMD Millipore serves as a strategic partner to customers and helps advance the promise of life science. Headquartered in Billerica, Massachusetts, the division has around 10,000 employees, operations in 66 countries and 2013 revenues of €2.6 billion.

About Merck KGaA, Darmstadt, Germany
Merck KGaA of Darmstadt, Germany, is a leading company for innovative and top-quality high-tech products in the pharmaceutical and chemical sectors. Its subsidiaries in Canada and the United States operate under the umbrella brand EMD. Around 39,000 employees work in 66 countries to improve the quality of life for patients, to further the success of customers and to help meet global challenges. The company generated total revenues of € 11.1 billion in 2013 with its four divisions: Biopharmaceuticals, Consumer Health, Performance Materials and Life Science Tools. Merck KGaA, Darmstadt, Germany is the world’s oldest pharmaceutical and chemical company – since 1668, the name has stood for innovation, business success and responsible entrepreneurship. Holding an approximately 70 percent interest, the founding family remains the majority owner of the company to this day.