Chromolith® SpeedROD RP-18e

Column	Chromolith® SpeedROD RP-18 endcapped, 50-4.6 mm
Mobile phase	A: Acetonitrile B: 0.01 M Phosphate buffer pH 5.0
Gradient	Time/min	%A	%B
	0.0	3	97
	2.5	3	97
	2.6	8	92
	5.0	8	92
Flow rate	4 mL/min
Detection	227 nm
Temperature	Ambient
Injection volume	10 μl
Sample	1. Acesulfame-K (23 μg/mL) 2. Saccharin (29 μg/mL) 3. Benzoic acid (13 μg/mL) 4. Sorbic acid (14 μg/mL) 5. Caffeine (47 μg/mL) 6. Aspartame (100 μg/mL)