Chromolith® Performance RP-18e , (L) 100 mm for Rapid Separation of More Complex Mixtures

Column	Chromolith® Performance, 100-4.6 mm
Mobile phase	A: Acetonitrile B: 20 mM Phosphate buffer pH 4.5
Gradient	Time/min	%A	%B
	0.0	20	80
	3.0	60	40
Flow rate	4 mL/min
Detection	230 nm
Temperature	22°C
Injection volume	10 μl
Sample	1. Ascorbic acid (100 μg/mL) 2. 4-Hydroxybenzoic acid (100 μg/mL) 3. Benzoic acid (100 μg/mL) 4. Sorbic acid (150 μg/mL) 5. Methyl 4-hydroxybenzoate (100 μg/mL) 6. Ethyl 4-hydroxybenzoate ( μg/mL) 7. Propyl 4-hydroxybenzoate ( μg/mL)