Separation of Steroids


Column 2 columns of Chromolith® Performance RP-18 endcapped Separation of steroids
Mobile phase A: Acetonitrile
B: Water
Gradient Time/min %A %B
0.0 80
7.0 10
Flow rate 3.0 mL/min
Detection UV 220 nm
Temperature Ambient
Injection volume 10 μL
Sample

1. Prednisolone
2. Cortisone
3. Nortestosterone
4. Estradiol
5. Testosterone
6. Corticosterone
7. Estrone
8. Progesterone