Separation of Carbidopa

Column	Chromolith® Performance RP-18 endcapped, 100-4.6 mm
Mobile phase	A: Methanol B: 0.1% TFA in water
Gradient	Time/min	%B
	0.0	100
	1.0	100
	10	80
Flow rate	2 mL/min
Detection	UV 282 nm
Temperature	Ambient
Injection volume	5 μl
Sample	1. 2,4,5 Trihydroxyphenalalanine 2. Levodopa 3. Methyldopa 4. Dopamine 5. Carbidopa 6. 3,4-Dihydroxyphenylacetic acid 7. 3-o-Methylcarbidopa	125 μg/mL 235 μg/mL 160 μg/mL 190 μg/mL 175 μg/mL 185 μg/mL 105 μg/mL