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Immunology Assays for Muse® Cell Analyzer

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The Muse® system brings the essential immunology analyses you perform the most right to your bench top. Obtain rapid single cell analysis of total human CD4, CD8, and B cell counts, relative CD25 expression, and lymphocyte activation. Using optimized assays that eliminate the need for voltage detector setup, modules for each assay automatically calculate absolute cell counts and subpopulation ratios as percentages of total sample. No other cytometer offers quantitative results in less than an hour.

Immunology Assays

Muse® Human CD4 T Cell Kit

(Cat. No. MIM100101)

The Muse® Human CD4 T Cell Assay allows you to determine the concentration and percentages of CD4 T cells and lymphocytes in human whole blood or peripheral blood mononuclear cells (PBMCs). Characterizing CD4 T cell counts and percentages is important in evaluating immune status, understanding disease mechanism, in vaccine research and immunotoxicity studies. The Muse® Human CD4 T Cell Kit allows for the detection and identification of lymphocytes and CD4 T lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. The CD4 antibody identifies human helper/inducer CD4+ T cell (HLA Class II reactive) and recognizes a 60,000 Da surface antigen. Monocytes also express CD4 but at a lower density, and have no co-expression of the other antibodies present in the anti-lymphocyte cocktail; hence can be distinguished from CD4 T Cells in this kit. The simplified identification can be of great utility in understanding the role of CD4 T lymphocytes in development, response, and mechanism of disease.

Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Muse® Cell Analyzer provides:
  • Count, percentage, and concentration of CD4 T cells
  • Count and concentration of lymphocytes
The Muse® Human CD4 T Cell Assay is for use with the Muse® Cell Analyzer. The Muse® System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse® Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps. Sufficient reagent is provided for the preparation and analysis of 100 tests.

CAUTION: This kit should not be used for patient diagnosis or patient management. For Research Use Only; not for use in diagnostic procedures.
  • Kit Components

    • Muse® Human CD4 T Cell Cocktail (Part No. 4700-1615, 100 tests/vial)
    • Human 1X Lysing Solution (Part No. 4700-1620, 100 tests/vial)
    • 1X Assay Buffer BA (Part No. 4700-1360, 100 tests/vial)
  • Materials Recommended

    • Muse® Cell Analyzer
    • Whole blood or PBMC samples
    • Micropipettors
    • Disposable micropipettor tips
    • Microcentrifuge tubes with screw caps, 1.5 mL (VWR, Catalog No. 16466-030, or equivalent)
    • Vortex mixer
    • Disposable gloves
    • 100% bleach solution
    • Deionized water
    • Muse® System Check Kit (Catalog No. MCH100101)
    • Muse® Count & Viability Reagent (Catalog No. MCH100102)
    • Guava Fixative (Catalog No. 4700-0140), optional
    • Guava ICF Instrument Cleaning Fluid (Catalog No. 4200-0140), optional
  • Expected Results

    Figures A and B show typical results obtained with the Muse® CD4 T Cell Kit

    Figures A and B. Whole blood was stained with the Muse® Human CD4 T Cell Kit and acquired on the Muse® Cell Analyzer. Figure A shows summary data, while Figure B shows results displayed with optional dot plots. The statistics show the percentages and the concentrations (cells/μL) for the results in each quadrant out of the total events. The Lymphocyte Results show the total lymphocyte concentration, the CD4 T-lymphocyte concentration, and the CD4 cell percentage of lymphocytes. The first plot in Figure B shows Cell Size Index vs Lymphocytes and a lymphocyte gate, and the second plot shows CD4 vs Lymphocytes.




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Muse® Human CD8 T Cell Kit

(Cat. No. MIM100102)

The Muse® Human CD8 T Cell Kit is used in laboratory research studies to determine the CD8 T-cell count, total lymphocyte count, and CD 8 T-cell percent of lymphocytes in human whole blood and peripheral mononuclear cell (PBMC) samples. Identification and enumeration of cytotoxic CD8 T-cell populations are useful in understanding immune response and status, and in the research of disease mechanisms, such as autoimmune diseases, immunodeficiency.

The Muse® Human CD8 T Cell Kit allows for the detection and identification of lymphocytes and CD8 T lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. The CD8 antibody allows the identification of CD8, a 68-kDa transmembrane glycoprotein expressed by class I major histocompatibility complex restricted, mature suppressor/cytotoxic T cells, the great majority of cortical thymocytes and approximately 30% of medullary thymocytes. In addition a proportion of γδ T cells and NK cells express CD8. Inclusion of several proprietary antibodies in the anti-lymphocyte cocktail allows for the unique identification of the CD8 cytotoxic T cells.

Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Muse® Cell Analyzer provides:CD8 T-cell concentration in cells/μL

  • CD8 T-cell concentration in cells/μL
  • CD8 T-cell percent of lymphocytes
  • Total lymphocyte concentration in cells/μL
The Muse® Human CD8 T Cell Assay is for use with the Muse® Cell Analyzer. The Muse® System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse® Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps. Sufficient reagent is provided for the preparation and analysis of 100 tests.

CAUTION:This kit should not be used for patient diagnosis or patient management. For Research Use Only; not for use in diagnostic procedures.
  • Kit Components

    • Muse® Human CD8 T Cell Cocktail (Part No. 4700-1625, 100 tests/vial)
    • Human 1X Lysing Solution (Part No. 4700-1620, 100 tests/vial)
    • 1X Assay Buffer BA (Part No. 4700-1360, 100 tests/vial)
  • Materials Recommended

    • Muse® Cell Analyzer
    • Whole blood or PBMC samples
    • Micropipettors
    • Disposable micropipettor tips
    • Microcentrifuge tubes with screw caps, 1.5 mL (VWR, Catalog No. 16466-030, or equivalent)
    • Vortex mixer
    • Disposable gloves
    • 100% bleach solution
    • Deionized water
    • Muse® System Check Kit (Catalog No. MCH100101)

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Muse® Human B Cell Kit

(Cat. No. MIM100103)

The Muse® Human B Cell Kit is for use in laboratory research studies to determine the B cell count, total lymphocyte count, and B cell percent of lymphocytes in human whole blood and peripheral blood mononuclear cell (PBMC) samples. The characterization of B cell counts and percentage is important in multiple areas, such as understanding humoral immune response, research on mechanisms of autoimmune diseases, factors that influence T-cell responses, and for improved vaccine development.

The Muse® Human B Cell Kit detects and identifies lymphocytes and CD19 B lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. CD19 is considered to be a characteristic B cell marker and therefore commonly used in the routine immunophenotyping of B cells. Monoclonal antibodies clustered as CD19 detect all peripheral blood B cells.4 The simplified identification can be of great utility in understanding the role of B lymphocytes in development, response, and mechanism of disease.

Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Muse® Cell Analyzer provides:
  • Count, percentage, and concentration of B cells
  • Count and concentration of lymphocytes
The Muse® Human B Cell Assay is for use with the Muse® Cell Analyzer. The Muse® System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse® Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps.

Sufficient reagent is provided for the preparation and analysis of 100 tests.

CAUTION: This kit should not be used for patient diagnosis or patient management. For Research Use Only; not for use in diagnostic procedures.
  • Kit Components

    • Muse® Human B Cell Cocktail (Part No. 4700-1635, 100 tests/vial)
    • Human 1X Lysing Solution (Part No. 4700-1620, 100 tests/vial)
    • 1X Assay Buffer BA (Part No. 4700-1360, 100 tests/vial)
  • Materials Recommended

    • Muse® Cell Analyzer
    • Whole blood or PBMC samples
    • Micropipettors
    • Disposable micropipettor tips
    • Microcentrifuge tubes with screw caps, 1.5 mL (VWR, Catalog No. 16466-030, or equivalent)
    • Vortex mixer
    • Disposable gloves
    • 100% bleach solution
    • Deionized water
    • Muse® System Check Kit (Catalog No. MCH100101)
    • Muse® Count & Viability Reagent (Catalog No. MCH100102)
    • Guava Fixative (Catalog No. 4700-0140), optional
    • Guava ICF Instrument Cleaning Fluid (Catalog No. 4200-0140), optional
  • Expected Results

    Figures A and B show typical results obtained with the Muse® Human B Cell Kit.

    Figures A and B. Whole blood was stained with the Muse® Human B Cell Kit and acquired on the Muse® Cell Analyzer. Figure A shows summary data, while Figure B shows results displayed with optional dot plots. The statistics show the percentages and the concentrations (cells/μL) for the results in each quadrant out of the total events. The Lymphocyte Results show the total lymphocyte concentration, the B lymphocyte concentration, and the B cell percentage of lymphocytes. The first plot in Figure B shows Cell Size Index vs Lymphocytes and a lymphocyte gate, and the second plot shows CD19 vs Lymphocytes.




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Muse® Human Lymphocyte CD69 Kit

(Cat. No. MIM100104)

The Muse® Human Lymphocyte CD69 Kit is for use in laboratory research studies to determine the CD69(+) lymphocyte count, total lymphocyte count, and CD69 percent of total lymphocytes in human whole blood and peripheral blood mononuclear cell (PBMC) samples. CD69 is an early-activation marker expressed on the surface of activated lymphocytes including T and B lymphocytes and NK cells. The study of CD69 expression on lymphocytes is important in understanding immune response and development, in immuno-modulatory mechanisms and in the research of diseases. The Muse® Human Lymphocyte CD69 Kit detects and identifies lymphocytes and CD69 lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. The Muse® Human Lymphocyte CD69 Assay uses an antilymphocyte cocktail that identifies the total lymphocyte population and a CD69 antibody that binds to the CD69 early activation marker expressed on lymphocytes. The kit also includes an isotype control antibody.

Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Muse® Cell Analyzer provides:
  • CD69(+) lymphocyte concentration in cells/μL .
  • CD69 percent of total lymphocytes.
  • Total lymphocyte concentration in cells/μL.
The Muse® Human Lymphocyte CD69 Assay is for use with the Muse® Cell Analyzer. The Muse® System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse® Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps.
  • Kit Components

    • Muse®Human Lymphocyte Cocktail (Part No. 4700-1650, 100 tests/vial)
    • Muse®Human CD69-PE Antibody (Part No. 4700-1655, 100 tests/vial)
    • Isotype Control Mouse IgG1-PE (Part No. 4700-1390, 100 tests/vial)
    • Human 1X Lysing Solution (Part No. 4700-1620, 100 tests/vial)
    • 1X Assay Buffer BA (Part No. 4700-1360, 100 tests/vial)
  • Materials Recommended

    • Muse®Cell Analyzer
    • Whole blood or PBMC samples
    • Micropipettors
    • Disposable micropipettor tips
    • Microcentrifuge tubes with screw caps, 1.5 mL (VWR, Catalog No. 16466-030, or equivalent)
    • Vortex mixer
    • Disposable gloves
    • 100% bleach solution
    • Deionized water
    • Muse®System Check Kit (Catalog No. MCH100101)
    • Muse®Count & Viability Reagent (Catalog No. MCH100102)
    • Guava Fixative (Catalog No. 4700-0140), optional
    • Guava ICF Instrument Cleaning Fluid (Catalog No. 4200-0140), optional
  • Expected Results

    Figures A and B show typical results obtained with the Muse® Human Lymphocyte CD69 Cell Kit Figures A and B. Whole blood was stained with the Muse® Human Lymphocyte CD69 Kit and acquired on the Muse® Cell Analyzer. Figure A shows summary data, while Figure B shows results displayed with optional dot plots. The statistics show the percentages and the concentrations (cells/μL) for the events in each quadrant out of the total events. Additionally, the Lymphocyte Results show the total lymphocyte concentration, the CD69(+) lymphocyte concentration, and the CD69 percentage of total lymphocytes. The first plot in Figure B shows Cell Size Index vs Lymphocytes and a lymphocyte gate, and the second plot shows CD69 vs Lymphocytes).




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Muse® Human Lymphocyte CD25 Kit

(Cat. No. MIM100105)

The Muse® Human Lymphocyte CD25 Kit is for use in laboratory research studies to determine the CD25 lymphocyte count, total lymphocyte count, and CD25 percent of total lymphocytes in human whole blood and PBMC samples. CD25 (IL-2 receptor á-chain) is an activation marker expressed on the surface of activated lymphocytes including T and B lymphocytes and NK cells. It is also expressed constitutively on the surface of small sub-populations of T cells. The study of CD25 expression on lymphocytes is important in understanding immune response and development, in immuno-modulatory mechanisms, and in the research of disease mechanisms. The Muse® Human Lymphocyte CD25 Kit detects and identifies total lymphocytes and CD25 lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. The assay includes an antilymphocyte cocktail that identifies the total lymphocyte population and a CD25 antibody that identifies mid to late activation marker CD25.

Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Muse® Cell Analyzer provides:
  • CD25(+) lymphocyte concentration in cells/μL.
  • CD25 percent of total lymphocytes.
  • Total lymphocyte concentration in cells/μL.
The Muse® Human Lymphocyte CD25 Assay is for use with the Muse® Cell Analyzer. The Muse® System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse® Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps.
  • Kit Components

    • Muse® Human Lymphocyte Cocktail (Part No. 4700-1650, 100 tests/vial).
    • The Muse® Human CD25-PE Antibody (Part No. 4700-1660, 100 tests/vial).
    • Isotype Control Mouse IgG1-PE (Part No. 4700-1390, 100 tests/vial).
    • Human 1X Lysing Solution (Part No. 4700-1620, 100 tests/vial).
    • 1X Assay Buffer BA (Part No. 4700-1360, 100 tests/vial).
  • Materials Recommended

    • Muse®Cell Analyzer
    • Whole blood or PBMC samples.
    • Disposable micropipettor tips.
    • Microcentrifuge tubes with screw caps, 1.5 mL (VWR, Catalog No. 16466-030, or equivalent).
    • Vortex mixer.
    • Disposable gloves.
    • 100% bleach solution
    • Deionized water
    • Muse®System Check Kit (Catalog No. MCH100101).
    • Muse®Count & Viability Reagent (Catalog No. MCH100102.
    • Guava Fixative (Catalog No. 4700-0140), optional.
    • Guava ICF Instrument Cleaning Fluid (Catalog No. 4200-0140), optional.
  • Expected Results

    Figures A and B show typical results obtained with the Muse® Human Lymphocyte CD25 Cell Kit Whole blood was stained with the Muse® Human Lymphocyte CD25 Kit and acquired on the Muse® Cell Analyzer. Figure A shows summary data, while Figure B shows results displayed with optional dot plots. The statistics show the percentages and the concentrations (cells/μL) for the events in each quadrant out of the total events. Additionally, the Lymphocyte Results show the total lymphocyte concentration, the CD25(+) lymphocyte concentration, and the CD25 percentage of total lymphocytes. The first plot in Figure B shows Cell Size Index vs Lymphocytes and a lymphocyte gate, and the second plot shows CD25 vs Lymphocytes.




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