Chromolith® HR RP-18 Endcapped

Speed, Packing Shape & Reproducibility

Chromolith® HighResolution RP-18 endcapped columns are the ultimate choice for faster chromatographic separations. The short monolithic structure of these HPLC columns delivers ultra-fast results at low back pressure for both isocratic and gradient applications. This allows high sample throughput and fast column re-equilibration between analyses, saving time and costs in your chromatographic analyses.

Chromolith® Performance and Chromolith® HighResolution in Comparison 

Chromolith® HighResolution RP-18e, 100-4.6 mm

Solvents	A: ACN B: 0.02 mM phosphate buffer pH 2.5
Gradient	0.0 min 20% A
Flow rate	3.5 ml/min
Column pressure	49-71 bar
Temperature	ambient
LCF system	LaChrom L7000
Detection	UV 230 nm
Vol. detector cell	16 µl
Inj. volume	Silica monolithic: 40 bar
Sample	1. Atenolol 2. Pindolol 3. Metoprolol 4. Bisoprolol 5. Labetalol 6. Propanolol

Chromolith® HighResolution RP-18e, 25-4.6mm

Solvents	60%  ACN / 40% water isocratic
Gradient	0.3 min 40% A
Flow rate	2.0 ml/min
Column pressure	15 bar
Temperature	ambient
LCF system
Detection	UV 254 nm
Vol. detector cell	16 µl
Inj. volume	Core-shell: 100 bar
Sample	1. Thiourea 2. Biphenyl-2-ol 3. Progesterone 4. Hexanophenone 5. Anthracene

Improved Peak Shape of Basic Compounds

Chromolith® HighResolution RP-18 endcapped stationary phases enable the elution of basic compounds with excellent peak shape and no tailing.

See Details

Mobile phase	A: ACN, B: 20 nM NaH2PO4 buffer pH 7.6
Gradient	0.0 min 20% A, 0.5 min 45% A
Flow rate	2.0 ml/min
Column pressure	63-69 bar
Temperature	ambient
Detection	UV 254 nm
Temperature	ambient
Vol. detector cell	16 µl
Inj. volume	10 µL
Sample	1. Caffeine 2. Aniline 3. N-Methylaniline 4. 2-Ethylaniline 5. 4-Nitranisole 6. N,N-Dimethylaniline

Excellent Batch-to-Batch Reproducibility
Chromolith HighResolution RP-18e, 100-4.6 mm

The batch-to-batch reproducibility of Chromolith® HPLC columns is strictly monitored and fulfills the requirements of QA and QC laboratories.

See Details

Mobile phase	A: Acetonitrile + 0.1% TFA B: Water + 0.1% TFA
Gradient	2.0 min 0% A, 10.0 min 30% A
Flow rate	1.0 ml/min
Temperature	25°C
Detection	UV 210 nm
Inj. volume	2 µl
Sample	1. Norepinephrine 2. Octopamine 3. Epinephrine tartrate 4. Dopamine 5. DOPA 6. Norephedrine 7. Ephedrine 8. N-Methylephedrine

Features and Benefits

• Column performance corresponds to sub 3 µm particle-packed columns and is at least 50% higher compared to standard Chromolith® columns
• Back pressure at least half that of particulate columns
• Minimum 30% longer column lifetime compared with particle-packed columns

Products

Resources

• Applications
• Comparison with core-shell material
• Benefits: Speed, peak shape, reproducibility