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Chromolith® HPLC Columns


Flow Programming

Chromolith® HPLC columns respond very quickly to changes in flow rate, giving you maximum flexibility in flow programming. Rates can be adjusted in mid-flow either to enhance the peak definition of the target compound, or to shorten the total separation time once the target compound has successfully eluted. This enables clearer separation of two closely eluting peaks, without significantly affecting the total run time. A mid-flow change in flow rate can also reduce the total run time when certain compounds elute much later than other sample components.

Application Example

ColumnChromolith® Performance RP-18 endcapped, 100-4.6 mm

Mobile
phase

A: Acetonitrile
B: 0.1% Phosphoric acid in water
Double
gradient
Time
0 min
1.8 min
2.2 min
3 min
%A
35  
46
80
80
%B
65  
54
20
20
Flow rate
3 mL/min
3 mL/min
5 mL/min
5 mL/min
Pressure 90 bar maximum total pressure
Detection UV 254 nm
Temperature 22°C
Injection
volume
10 μL
Sample 1. Phenol
2. 2-Chlorophenol
3. 2-Nitrophenol
4. 2,4-Dinitrophenol
5. Chloro-3-methylphenol
6. 2,4-Dinitro-6-methylphenol
7. 2,4,6-Trichlorophenol
8. Pentachlorophenol
Application Example: Chromolith® Performance RP-18 endcapped, 100-4.6 mm
 
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