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Primary Mouse Embryonic Fibroblasts

MEF Feeder Cells for ES Cell Culture

PMEF feeder cells at the correct density

Many embryonic stem (ES) cell culture protocols use a monolayer of primary mouse embryo fibroblast (PMEF) feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Derived from day 13 embryos, these cells are frozen into individual vials, containing approximately 5-6 x 10⁶ fibroblasts per vial, and are sold as a pack of 5 vials per kit at various passage number (P1-P3). Several varieties are available, including drug resistant, growth arrested, and not mitomycin-C treated feeder cells.

PMEF-CF1:

PMEF cells isolated from CF-1 mouse embryos at day 13. Most commonly used PMEF cell line used to support mouse and human embryonic stem cells in culture.

PMEF-N:

Neomycin resistant PMEF cells isolated from FVB mouse embryos at day 13. Commonly used in genetic selection experiments using neomycin as selection agent.

PMEF-H:

Hygromycin resistant PMEF cells isolated from C57BL/6 mouse embryos at day 13.

PMEF-DR4:

DR4 MEF feeder cells are resistant to neomycin, hygromycin, puromycin, and 6-thioguanine.

PRODUCT DESCRIPTION	TREATMENT	PASSAGE	QTY/PK	CAT. NO.
EmbryoMax® Primary Mouse Embryo Fibroblasts Strain CF1	Not Treated	P1	1 vial, 1 x 10⁶ cells ea.	PMEF-CFL-P1
	Not Treated	P3	5 vials, 5-6 x 10⁶ cells ea.	PMEF-CFL
	Mitomycin-C Treated	P3	5 vials, 5-6 x 10⁶ cells ea.	PMEF-CF
	Irradiated	P3	5 vials, 5-6 x 10⁶ cells ea.	PMEF-CFX
EmbryoMax® Primary Mouse Embryo Fibroblasts Neo Resistant	Not Treated	P1	1 vial, 1 x 10⁶ cells ea.	PMEF-NL-P1
	Not Treated	P3	5 vials, 5-6 x 10⁶ cells ea.	PMEF-NL
	Mitomycin-C Treated	P3	5 vials, 5-6 x 10⁶ cells ea.	PMEF-N
	Irradiated	P3	5 vials, 5-6 x 10⁶ cells ea.	PMEF-NX
EmbryoMax® Primary Mouse Embryo Fibroblasts Hygro Resistant	Not Treated	P3	5 vials, 5-6 x 10⁶ cells ea.	PMEF-HL
EmbryoMax® Primary Mouse Embryo Fibroblasts Hygro Resistant	Mitomycin-C Treated	P3	5 vials, 5-6 x 10⁶ cells ea.	PMEF-H
EmbryoMax® Primary Mouse Embryo Fibroblasts Strain DR4	Mitomycin-C Treated	P3	1 vials, 4 x 10⁶ cells ea.	PMEF-DR4
	Mitomycin-C Treated	P3	5 vials, 4 x 10⁶ cells ea.	PMEF-DR4-5P
	Irradiated	P3	1 vials, 4 x 10⁶ cells ea.	PMEF-DR4X
	Irradiated	P3	5 vials, 4 x 10⁶ cells ea.	PMEF-DR4X-5P

Learn more about PMEF cells

Interested in purchasing an entire lot of PMEF’s? Bulk sized lots of PMEF are available with lot sampling.

Contact your local MilliporeSigma account manager or MilliporeSigma customer service to set up a bulk order.

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