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UCOE® References - Mammalian Expression

Article	Application	Cell Type	UCOE element used	Protein Expressed
Correlation of DNA methylation with histone modifications across the HNRPA2B1-CBX3 ubiquitously-acting chromatin open element (UCOE). Lindahl Allen M, Antoniou M. Epigenetics. 2007 Oct-Dec;2(4):227-36.	Basic mechanisms	Human peripheral blood mononuclear cells (PBMCs)	5kb human HNRPA2B1-CBX3 region	N/A
Epigenetic regulatory elements associate with specific histone modifications to prevent silencing of telomeric genes. Majocchi S, Aritonovska E, Mermod N. Nucleic Acids Res. 2014 Jan;42(1):193-204.	Basic mechanisms	HeLa	1.5kb human HNRPA2B1	N/A
Integrative analysis of methylome and transcriptome reveals the importance of unmethylated CpGs in non-CpG island gene activation. Marx A, Kahan T, Simon I. Biomed Res Int. 2013 2013:785731.	Basic mechanisms	N/A	N/A	N/A
Transcriptional Regulation of the Human TATA Binding Protein Gene. Harland L, Crombie R, Anson S, deBoer J, Ioannou PA, Antoniou M. Genomics. 2002 Apr;79(4):479-82.	Basic mechanisms	Murine L-cells	44 kb human TBP region	N/A
Transgenes encompassing dual-promoter CpG islands from the human TBP and HNRPA2B1 loci are resistant to heterochromatin-mediated silencing. Antoniou M, Harland L, Mustoe T, Williams S, Holdstock J, Yague E, Mulcahy T, Griffiths M, Edwards S, Ioannou PA, M	Basic mechanisms	Murine L-cells CHO	25 kb human TBP; 16 kb human HNRPA2B1	EGFP
Interplay of Promoter Usage and Intragenic CpG Content: Impact on GFP Reporter Gene Expression. Krinner S, Heitzer A, Asbach B, Wagner R. Hum Gene Ther. 2015 Dec;26(12):826-40. doi: 10.1089/hum.2015.075. Epub 2015 Sep 29.	Bioprocessing	Flp-In CHO P19	2.2 kb human HNRPA2B1; expression from HNRPA2B1 promoter	EGFP
Assessment of UCOE on Recombinant EPO Production and Expression Stability in Amplified Chinese Hamster Ovary Cells. Betts Z, Dickson AJ. Mol Biotechnol. 2015 Sep;57(9):846-58. doi: 10.1007/s12033-015-9877-y.	Bioprocessing	CHO-DG44	8kb human HNRPA2B1 linked upstream of hCMV promoter	EPO
Chromatin Function Modifying Elements in an Industrial Antibody Production Platform - Comparison of UCOE, MAR, STAR and cHS4 Elements. Saunders F, Sweeney B, Antoniou MN, Stephens P, Cain K. PLoS One. 2015 Apr 7;10(4):e0120096.	Bioprocessing	CHO-K1	1.5kb human HNRPA2B1 linked upstream of hCMV promoter	mAb
CpG-island fragments from the HNRPA2B1/CBX3 genomic locus reduce silencing and enhance transgene expression from the hCMV promoter/enhancer in mammalian cells. Williams S, Mustoe T, Mulcahy T, Griffiths M, Simpson D, Antoniou M, Irvine A, Mountain A, Crom	Bioprocessing	CHO-K1	1.5 kb 4 kb 8 kb human HNRPA2B1, all linked upstream of hCMV promoter	EGFP EPO
Effect of different UCOE-promoter combinations in creation of engineered cell lines for the production of Factor VIII. Nair AR, Jinger X, Hermiston TW. BMC Res Notes. 2011 Jun 10;4:178.	Bioprocessing	BHK21	1.5 kb human HNRPA2B1; 3.2 kb mouse Rps3; linked upstream of hCMV	B domain deleted factor VIII (BDD-FVIII)
Evaluating the interaction between UCOE and DHFR-linked amplification and stability of recombinant protein expression. Betts Z, Croxford AS, Dickson AJ. Biotechnol Prog. 2015 Jul-Aug;31(4):1014-25. doi: 10.1002/btpr.2083. Epub 2015 Apr 8.	Bioprocessing	CHO-DG44	8 kb human HNRPA2B1 upstream of hCMV	EGFP
High-throughput ClonePix FL analysis of mAb-expressing clones using the UCOE expression system. Hou JJ, Hughes BS, Smede M, Leung KM, Levine K, Rigby S, Gray PP, Munro TP. N Biotechnol. 2014 May 25;31(3):214-20.	Bioprocessing	CHO-K1 (CHO XL-99)	3.2 kb mouse Rps3, 8 kb human HNRPA2B1; linked upstream of gpCMV & hCMV	mAb
Rapid protein production using CHO stable transfection pools. Ye J, Alvin K, Latif H, Hsu A, Parikh V, Whitmer T, Tellers M, de la Cruz Edmonds MC, Ly J, Salmon P, Markusen JF. Biotechnol Prog. 2010 Sep-Oct;26(5):1431-7.	Bioprocessing	CHO-K1SV	3.2 kb mouse Rps3, linked upstream of hCMV	Model Abs
Simple scale-up of recombinant antibody production using an UCOE containing vector. Boscolo S, Mion F, Licciulli M, Macor P, De Maso L, Brce M, Antoniou MN, Marzari R, Santoro C, Sblattero D. N Biotechnol. 2012 May 15;29(4):477-84.	Bioprocessing	CHO-S	4 kb human HNRPA2B1, linked upstream of hCMV	Dipeptil peptidase 6 - DPP6 (human) Transglutaminase 2 - TG2 (human) TRAIL R2 (human) CD55 Complement 5
Stable expression of H1C2 monoclonal antibody in NS0 and CHO cells using pFUSE and UCOE expression system. Dharshanan S, Chong H, Cheah SH, Zamrod Z. Cytotechnology. 2013 Jul 24.	Bioprocessing	NS0 CHO	3.2 kb mouse Rps3, linked upstream of hCMV	humanized anti-C2 (H1C2 mAb)
The use of UCOE vectors in combination with a preadapted serum free, suspension cell line allows for rapid production of large quantities of protein. Benton T, Chen T, McEntee M, Fox B, King D, Crombie R, Thomas TC, Bebbington C. Cytotechnology. 2002 Jan;	Bioprocessing	CHO-K1 CHO-S	8kb human HNRPA2B1, linked upsteam of hCMV	GFP humanized mAb
Ubiquitous Chromatin Opening Elements (UCOEs) effect on transgene position and expression stability in CHO cells following methotrexate (MTX) amplification. Betts Z and Dickson AJ. Biotechnol J. 2015 Dec 3. doi: 10.1002/biot.201500159. [Epub ahead of prin	Bioprocessing	CHO-DG44	8 kb human HNRPA2B1, linked upstream of hCMV	EGFP
Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors. Bandaranayake AD1, Correnti C, Ryu BY, Brault M, Strong RK, Rawlings DJ. Nucleic Acids Res.	Bioprocessing	293 Freestyle; CHO-S	2kb human, 0.7 kb human HNRPA2B1; linked upstream of SFFV	Siderocalin (human & mouse) Lipocalin 15 (human) Glycodelin (human) MICA (soluble) MICA alpha 3 CARMA-CARD 4E10 5B6 IL-3 (canine) G-CSF (canine) IL-7 (human) LIF (human); EGFP
A ubiquitous chromatin opening element (UCOE) confers resistance to DNA methylation-mediated silencing of lentiviral vectors. Zhang F, Frost AR, Blundell MP, Bales O, Antoniou MN, Thrasher AJ. Mol Ther. 2010 Sep;18(9):1640-9.	Lentivirus	Murine P19 embryonal carcinoma Murine HSC	2.2kb human HNRPA2B1; expression from HNRPA2B1 promoter; 1.5/1.2kb human HNRPA2B1 linked upstream of SFFV promoter	EGFP
Correction of murine Rag2 severe combined immunodeficiency by lentiviral gene therapy using a codon-optimized RAG2 therapeutic transgene. van Til NP, de Boer H, Mashamba N, Wabik A, Huston M, Visser TP, Fontana E, Poliani PL, Cassani B, Zhang F, Thrasher	Lentivirus	Murine HSC targetted; Rag2 KO mice; effects measured in peripheral blood mononuclear cells	2.2kb human HNRPA2B1; expression from HNRPA2B1 promoter	Rag2 protein
Lentiviral MGMT(P140K)-mediated in vivo selection employing a ubiquitous chromatin opening element (A2UCOE) linked to a cellular promoter. Phaltane R, Lachmann N, Brennig S, Ackermann M, Modlich U, Moritz T. Biomaterials. 2014 Aug;35(25):7204-13.	Lentivirus	Murine HSC; effects measured in peripheral blood cells	1.5kb human HNRPA2B1, linked upstream of murine PGK promoter	MGMT, EGFP
Lentiviral vectors containing an enhancer-less ubiquitously acting chromatin opening element (UCOE) provide highly reproducible and stable transgene expression in hematopoietic cells. Zhang F, Thornhill SI, Howe SJ, Ulaganathan M, Schambach A, Sinclair J,	Lentivirus	K562 Jurkat HeLa ED-7R Murine HSC	2.2 kb human HNRPA2B1; expression from HNRPA2B1 promoter	EGFP Luciferase IL2RG
Long-Term Reproducible Expression in Human Fetal Liver Hematopoietic Stem Cells with a UCOE-Based Lentiviral Vector. Dighe N, Khoury M, Mattar C, Chong M, Choolani M, Chen J, Antoniou MN, Chan JK. PLoS One. 2014 Aug 12;9(8).	Lentivirus	Human fetal liver-derived HSC (hflHSC)	2.2 kb human HNRPA2B1; expression from HNRPA2B1 promoter	EGFP
Physiological regulation of transgene expression by a lentiviral vector containing the A2UCOE linked to a myeloid promoter. Brendel C, Müller-Kuller U, Schultze-Strasser S, Stein S, Chen-Wichmann L, Krattenmacher A, Kunkel H, Dillmann A, Antoniou MN, Grez	Lentivirus	PLB985, PLB985 gp91phox^-/- P19 Raji Jurkat HUT-78; murine HSC	1.5kb human HNRPA2B1, linked upstream of MRP8 promoter	gp91phox EGFP
The human desmin promoter drives robust gene expression for skeletal muscle stem cell-mediated gene therapy. Jonuschies J, Antoniou M, Waddington S, Boldrin L, Muntoni F, Thrasher A, Morgan J. Curr Gene Ther. 2014 14(4):276-288.	Lentivirus	Murine C2C12 myoblasts; H-2Kb-tsA58-Myf5nLacZ/+ conditionally immortalised murine myoblasts; primary murine skeletal muscle satellite cells; human immortalised myoblasts	2.2kb human HNRPA2B1; expression from HNRPA2B1 promoter; 1.5kb human HNRPA2B1 linked upstream of human Desmin promoter	GFP
A minimal ubiquitous chromatin opening element (UCOE) effectively prevents silencing of juxtaposed heterologous promoters by epigenetic remodeling in multipotent and pluripotent stem cells. Müller-Kuller U, Ackermann M, Kolodziej S, Brendel C, Fritsch J,	Lentivirus	P19 Mouse Lin^- HSC mESC CCE hCD34iPSC11 (iPSC) PLB985 Jurkat cells	1.5 kb/674bp human HNRPA2B1 linked upstream of SFFV & MRP8; 674 bp human CBX3; expression from CBX3 promoter	EGFP
A ubiquitous chromatin opening element prevents transgene silencing in pluripotent stem cells and their differentiated progeny. Pfaff N, Lachmann N, Ackermann M, Kohlscheen S, Brendel C, Maetzig T, Niemann H, Antoniou MN, Grez M, Schambach A, Cantz T, Mor	Lentivirus	Murine iPSC-7 Human hES3	1.5 kb human HNRPA2B1, linked upstream of SFFV, EF1a	Human cytidine daminase (CDD) dTomato
Biphasic modulation of Wnt signalling supports efficient foregut endoderm formation from human pluripotent stem cells. Hoepfner J, Kleinsorge M, Papp O, Ackermann M, Alfken S, Rinas U, Solodenko W, Kirschning A, Sgodda M, Cantz T. Cell Biol Int. 2016 Feb	Lentivirus	H9 human ES cells hFLF-4 human iPS cells MEF primary murine embryonic fibroblasts	1.5 kb human HNRPA2B1, upstream of EF1a, TCF4/LEF1	dTomato Firefly luciferase
Promoter and lineage independent anti-silencing activity of the A2 ubiquitous chromatin opening element for optimized human pluripotent stem cell-based gene therapy. Ackermann M, Lachmann N, Hartung S, Eggenschwiler R, Pfaff N, Happle C, Mucci A, Göhring	Lentivirus	miPSC line lin-7 hCD34-derived iPS cells H9 human ES cells	1.5 kb human HNRPA2B1, upstream of EF1a, SFFV	dTomato human cytidine daminase (CDD)
Desmin-regulated Lentiviral Vectors for Skeletal Muscle Gene Transfer. Talbot GE, Waddington SN, Bales O, Tchen RC, Antoniou MN. Mol Ther. 2010 Mar;18(3):601-8.	Lentivirus	L cells, murine fibroblasts; C2C12, murine myoblasts MF1 mouse skeletal muscle	1.5 kb human HNRPA2B1, upstream of human Desmin promoter	EGFP
Lentiviral vector transduction of spermatozoa as a tool for the study of early development. Chandrashekran A, Isa I, Dudhia J, Thrasher AJ, Dibb N, Casimir C, Readhead C, Winston R. FEBS Open Bio. 2014 Mar 3;4:266-75.	Lentivirus	Boar spermatozoa B6/CBA1 mouse sperm	2.5 kb human HNRPA2B1; expression from HNRPA2B1 promoter	EGFP
Safer, Silencing-Resistant Lentiviral Vectors: Optimization of the Ubiquitous Chromatin-Opening Element through Elimination of Aberrant Splicing. Knight S, Zhang F, Mueller-Kuller U, Bokhoven M, Gupta A, Broughton T, Sha S, Antoniou MN, Brendel C, Grez M,	Lentivirus	Bcl-15, murine B-cell lymphoma; PLB-985, human myelomonocytic cells; P19	2.2 kb human HNRPA2B1; expression from HNRPA2B1 promoter; 1.5 kb human HNRPA2B1, upstream of PGK, MRP8 promoters	IL-2RG EGFP
Foamy virus vector-mediated gene correction of a mouse model of Wiskott-Aldrich syndrome. Uchiyama T, Adriani M, Jagadeesh GJ, Paine A, Candotti F. Mol Ther. 2012 Jun;20(6):1270-9.	Other vectors	Murine HSC; WAS KO mice; effects measured in peripheral blood mononuclear cells	631 bp human HNRPA2B1; expression from HNRPA2B1 promoter	WAS protein EGFP
Optimization of short-term transgene expression by sodium butyrate and ubiquitous chromatin opening elements (UCOEs). de Poorter JJ, Lipinski KS, Nelissen RG, Huizinga TW, Hoeben RC. J Gene Med. 2007 Aug;9(8):639-48.	Other vectors	Diploid human interface cells	1.5kb human HNRPA2B1, linked upstream of hCMV	Luciferase
Gene Expression Profile and Toxic Effects in Human Bronchial Epithelial Cells Exposed to Zearalenone. So MY, Tian Z, Phoon YS, Sha S, Antoniou MN, Zhang JW, Wu RS, Tan-Un KC. PLoS One. 2014 May 2;9(5).	Other vectors	BEAS-2B (human bronchial epithelial cells)	1.5 kb human HNRPA2B1, linked upstream of hCMV	Cytoglobin (Cygb)
Genomic cis-acting Sequences Improve Expression and Establishment of a Nonviral Vector. Hagedorn C, Antoniou MN, Lipps HJ. Mol Ther Nucleic Acids. 2013 Sep 3;2:e118.	Other vectors	CHO	1.5 kb human HNRPA2B1, linked upstream of hCMV	EGFP
Ubiquitous expression of the rtTA2S-M2 inducible system in transgenic mice driven by the human hnRNPA2B1/CBX3 CpG island. Katsantoni EZ, Anghelescu NE, Rottier R, Moerland M, Antoniou M, de Crom R, Grosveld F, Strouboulis J. BMC Dev Biol. 2007 Sep 27;7:10	Other vectors	Transgenic mice	8 kb human HNRPA2B1; expression from HNRPA2B1 promoter	Dox inducible rtTA2S-M2 Tet-transactivator
Optimizing retroviral gene expression for effective therapies. Antoniou MN, Skipper KA, Anakok O. Hum Gene Ther. 2013 Apr;24(4):363-74.	Review	N/A	N/A	N/A
Regulatory Elements in Vectors for Efficient Generation of Cell Lines Producing Target Proteins. Maksimenko O, Gasanov NB, Georgiev P. Acta Naturae. 2015 Jul-Sep;7(3):15-26.	Review	N/A	N/A	N/A

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