Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
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Protein levels were measured using the Direct Detect™ infrared-based quantitation system by spotting 2 µL of lysate on a Direct Detect™ Assay-free Card and inserting into the instrument. BugBuster® reagent with both Benzonase® nuclease and rLysozyme™ solution yielded lysates with the most 6XHis-CRP. Error bars represent standard deviation of triplicate measurements
When purifying proteins for functional or structural studies, the first step is to disrupt the cells or tissue sample and extract the relevant protein fraction. This step is critical because processing methods that require harsh mechanical, chemical, or enzymatic treatments can affect the target protein’s integrity and activity, or otherwise expose it to degradative conditions. MilliporeSigma’s complete range of reagents and enzymes for cell lysis and protein extraction provide you with an array of options so that you can put together the perfect extraction protocol for your particular cells and protein.
Reagents for Optimizing Your Protein Extraction Protocol:
