Agarose Beads – Recombinant Proteins |
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Chemical structure of Ni-NTA His•Bind® resin shows basis for high capacity binding of His-tagged proteins.
GST•Bind™ purification. A crude extract containing unfused GST was applied to a 2-mL GST•Bind™ Resin column. Total protein yield after purification was 8 mg/mL resin. Lane M is marker, lane 1 is BugBuster® extract, lane 2 is flow-through and lane 3 is eluate.
The GST•Bind™ purification system is based on the widely recognized affinity of glutathione-S-transferase (GST) fusion proteins for immobilized glutathione.
The Strep•Tag® II Kit contains the essential reagents for Strep•Tag® II fusion protein expression in E. coli and for fusion protein purification and detection.
Strep•Tactin® Superflow™ Agarose is a cross-linked agarose derivatized with Strep•Tactin® protein. It can be used for gravity flow as well as for low pressure and FPLC chromatography. Strep•Tactin® Superflow Agarose is optimized for column affinity chromatography as opposed to batch purification.
The T7•Tag® Antibody Agarose is designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag® sequence (i.e., the amino terminal 11 aa of the T7 gene 10 protein).
S-protein Agarose specifically retains S•Tag™ fusion proteins. Binding can be performed in a column or batch mode. Elution is possible using slightly denaturing conditions or using a specific protease, such as thrombin or enterokinase, with appropriate recombinant proteins.