Magnetic Beads - Recombinant Proteins |
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Advantages of PureProteome™ Nickel Magnetic Beads:
- Be efficient with high capacity nickel beads: bind 28 mg His-tagged protein/mL settled beads.
- High affinity nickel beads give you peace of mind: proteins bind beads tightly in buffers containing EDTA
- Achieve high purity and yields: low binding of untagged proteins yields highly pure His-tagged proteins
- Faster: shorten your protocol by 15 to 30 minutes
- Easier to use: clearly visible beads enable you to monitor each step.
RIGHT: Purification of 6X-His-tagged C-RP expressed in E. coli. 25 μL of settled beads (PureProteome™: 100 μL 20% slurry; Competitor G: 40 μL 50 % slurry) were washed in 10 mL binding buffer, then incubated with 500 μL E. coli lysate for 30 minutes at RT with end-over-end mixing. Beads were then washed three times with wash buffer containing 20 mM imidazole, then eluted with two fractions of 100 μL elution buffer containing either 250 mM imidazole (PureProteome™) or 500 mM imidazole (Competitor G). 10 μL of each sample was loaded.