Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

565764 γ-Secretase Substrate, Fluorogenic - Calbiochem

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: NMA-GGVVIATVK(DNP)-DRDRDR-NH₂

View Products on Sigmaaldrich.com
565764
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
565764-1MG Plastic ampoule 1 mg
Description
OverviewAn internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ40-, Aβ42-, and Aβ43-generating cleavage sites results in enhanced fluorescence.
Catalogue Number565764
Brand Family Calbiochem®
SynonymsNMA-GGVVIATVK(DNP)-DRDRDR-NH₂
References
ReferencesFarmery, M.R., et al. 2003. J. Biol. Chem. 278, 24277.
Product Information
ATP CompetitiveN
FormYellow Lyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₇₀H₁₁₆N₂₆O₁₈
Chemical formulaC₇₀H₁₁₆N₂₆O₁₈
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary Targetγ-secretase
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Peptide SequenceNMA-Gly-Gly-Val-Val-Ile-Ala-Thr-Val-Lys(DNP)-D-Arg-D-Arg-D-Arg-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot, flush with a stream of inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
565764-1MG 04055977267730

Documentation

γ-Secretase Substrate, Fluorogenic - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

γ-Secretase Substrate, Fluorogenic - Calbiochem Certificates of Analysis

TitleLot Number
565764

References

Reference overview
Farmery, M.R., et al. 2003. J. Biol. Chem. 278, 24277.

Citations

Title
  • Vorobyeva, A, et al. (2014) Cyclopamine Modulates Gamma-Secretase Mediated Cleavage of Amyloid Precursor Protein by Altering its Subcellular Trafficking and Lysosomal Degradation. J. Biol. Chemistry 289, 33258.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision10-September-2008 RFH
    SynonymsNMA-GGVVIATVK(DNP)-DRDRDR-NH₂
    DescriptionAn internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ40-, Aβ42-, and Aβ43-generating cleavage sites results in enhanced fluorescence. Excitation max.: ~355 nm; emission max.: ~440 nm.
    FormYellow Lyophilized
    FormulationSupplied as a trifluoroacetate salt.
    Intert gas (Yes/No) Packaged under inert gas
    Recommended reaction conditions50 mM Tris-HCl, pH 6.8, 2 mM EDTA, 0.25% CHAPSO (w/v), 8 µM substrate, 37°C.
    Chemical formulaC₇₀H₁₁₆N₂₆O₁₈
    Peptide SequenceNMA-Gly-Gly-Val-Val-Ile-Ala-Thr-Val-Lys(DNP)-D-Arg-D-Arg-D-Arg-NH₂
    Purity≥95% by HPLC
    SolubilityDMSO (5 mg/ml) or 5% Acetic acid
    Storage Protect from light
    -20°C
    Hygroscopic
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot, flush with a stream of inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
    Toxicity Standard Handling
    ReferencesFarmery, M.R., et al. 2003. J. Biol. Chem. 278, 24277.
    Citation
  • Vorobyeva, A, et al. (2014) Cyclopamine Modulates Gamma-Secretase Mediated Cleavage of Amyloid Precursor Protein by Altering its Subcellular Trafficking and Lysosomal Degradation. J. Biol. Chemistry 289, 33258.
    		•