Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

SCM112 3dGRO™ Wnt3a Conditioned Media Supplement

Alternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures.

SCM112
50 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCM112
Description3dGRO™ Wnt3a Conditioned Media Supplement
Alternate Names
  • Wnt3a Conditioned Media
  • Wnt3a CM
  • Wnt Conditioned Media
Background Information3D organoid culture systems are increasingly employed as powerful tools for the study of human diseases. Wnt proteins hetero-dimerize Frizzled (Fzd) family receptors and their co-receptors Lrp5/6, triggering downstream signaling pathways including the canonical β-catenin resulting in expression of genes regulating cell development. Wnt3a is considered an essential protein in many organoid culture systems (intestinal, pancreatic, liver, colon etc.) because the proliferation of stem cells is governed by the Wnt signaling pathway. However, due to the high concentration requirement in organoid media, the use of the recombinant Wnt3a becomes prohibitively expensive. Additionally, recombinant Wnt3a proteins are often unstable and lack biological activity. The 3dGRO™ Wnt3a Conditioned Media Supplement is a potent and inexpensive alternative to purified recombinant Wnt3a protein for organoid cultures and is optimized for the highest level of Wnt3a expression to ensure consistency and performance.

Note: Product is supplied at a 5X concentration (see datasheet for a complete organoid media recipe).

Source
The 3dGRO™ Wnt3a Conditioned Media Supplement is generated from mouse L-M(TK-) cells transfected with a mouse Wnt-3A expression vector. The conditioned medium contains fetal bovine serum.
References
Product Information
Applications
ApplicationAlternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures.
Key Applications
  • Cell Culture
  • Stem Cell Culture
Biological Information
Gene Symbol
  • WNT3A
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Appearance (color): clear, red liquid
• Endotoxin: <2 EU/mL
• Sterility Tested: No Growth/Pass
• Mycoplasma: Negative
• Media tested for function in supporting growth of human pancreatic organoids
• Media tested for biological activity using a Wnt3a luciferase reporter assay in HEK293 STF cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsWnt3a Conditioned Medium should be stored at -20°C. When ready to use, thaw the supplement overnight at 2-8°C in the dark. Thawed Wnt3a Conditioned Medium is good for two weeks when stored at 2-8°C.
Packaging Information
Material Size50 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
SCM112 04065269237051

Documentation