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14-840 Sigma-AldrichAMPK (α1, β1, γ1) Protein, active, 10 µg

Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

AMPK (α1, β1, γ1) Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA

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Overview

Replacement Information

Description
Catalogue Number	14-840
Brand Family	Upstate
Trade Name	Upstate
Description	AMPK (α1, β1, γ1) Protein, active, 10 µg
Overview	Recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1.

References

Product Information
Quality Level	MQ100

Applications
Application	Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.
Key Applications	Kinase Assay

Biological Information
Source	Expressed in E. coli cells and activated in vivo.
Specific Activity	For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number	AF100763 NM_006253 NM_002733
Entrez Gene Summary	The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene Symbol	PRKAB1 PRKAG1 PRKAA1
Protein Target	AMPK (α1, β1, γ1)
Purification Method	Cobalt-agarose
Target Sub-Family	CAMK
UniProt Number	Q13131, Q9Y478, P54619
Molecular Weight	AMPKα1 MW = 64 kDa, AMPKß1 MW = 30 kDa, AMPKγ1 MW = 38 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by phosphorylation of substrate peptide
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	6 months at -70°C

Packaging Information
Material Size	10 µg
Material Package	Also available in 250µg size as catalog # 14-840M - Call for pricing and availability.

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
14-840	04053252514395

Documentation

AMPK (α1, β1, γ1) Protein, active, 10 µg SDS

Title
Safety Data Sheet (SDS)

AMPK (α1, β1, γ1) Protein, active, 10 µg Certificates of Analysis

Title	Lot Number
AMPK (#945;1, #946;1, #947;1), active - 1767740-C	1767740-C
AMPK (#945;1, #946;1, #947;1), active - 1767740-D	1767740-D
AMPK (#945;1, #946;1, #947;1), active - 1767740-E	1767740-E
AMPK (#945;1, #946;1, #947;1), active - 1767740-F	1767740-F
AMPK (α1, β1, γ1), active - 1767740-G	1767740-G

Related Products & Applications

Product Families

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases

Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases

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