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GF165 ANG-2 Protein, Human Recombinant

Angiopoietin2 (Ang-2) is a secreted glycoprotein that plays a complex role in angiogenesis & inflammation.

ANG-2 Protein, Human Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: O15123
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GF165
20 µg  
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Overview

Replacement Information

Key Specifications Table

SourcePurity
CHO CellsProduct is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 95% pure by SDS-PAGE gel and HPLC analyses
Description
Catalogue NumberGF165
DescriptionANG-2 Protein, Human Recombinant
Alternate Names
  • ANG2
  • ANG
  • Angiopoietin 2
  • ANGPT2
Background InformationAngiopoietin2 (Ang-2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. ANG-2 binds to the endothelial cell specific receptor Tie2, but, in contrast to ANG-1 does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie2 and, depending on the physiological and biochemical environment, can act either as a n agonist or antagonist of Tie2 induced angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an α helix rich “coiled coil” N-terminal domain and fibrinogen like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer. Recombinant human ANG-2 is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 – 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein.
References
Product Information
PresentationProduct is filtered through a 0.2 micron filter before lyophilization.
Quality LevelMQ100
Applications
ApplicationAngiopoietin2 (Ang-2) is a secreted glycoprotein that plays a complex role in angiogenesis & inflammation.
Biological Information
PurityProduct is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 95% pure by SDS-PAGE gel and HPLC analyses
SourceCHO Cells
Cross ReactivityNone
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceBioactivity assay: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). Bioactivity assay: Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2μg/ml.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size20 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
GF165 04053252691225

Documentation

ANG-2 Protein, Human Recombinant Certificates of Analysis

TitleLot Number
Recombinant Human -2541572 2541572
Recombinant Human -2695301 2695301
Recombinant Human ANG-2 -3167162 3167162
Recombinant Human ANG-2 - 2135132 2135132
Recombinant Human ANG-2 - 2300022 2300022
Recombinant Human ANG-2 - 2515418 2515418
Recombinant Human ANG-2 - 3750000 3750000
Recombinant Human ANG-2 - 3788620 3788620
Recombinant Human ANG-2 - 3810156 3810156
Recombinant Human ANG-2 - 4046266 4046266
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