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14-339M Akt2/PKBβ (δPH, S474D) Protein, active, 250 µg

Active, recombinant human PKBβ, residues 120-481, S474D, containing an N-terminal His6 tag, activated by PDK1. For use in Kinase Assays.

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14-339M
250 µg  Also available in 10 μg size (cat#14-339) and in bulk (cat# 14-339-K).
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Overview

Replacement Information
Description
Catalogue Number14-339M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAkt2/PKBβ (δPH, S474D) Protein, active, 250 µg
OverviewRecombinant human PKBβ, residues 120-481, S474D, containing an N-terminal His6 tag; activated by PDK1.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, recombinant human PKBβ, residues 120-481, S474D, containing an N-terminal His6 tag, activated by PDK1. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
Gene Symbol
  • AKT2
  • RAC-BETA
  • PRKBB
  • PKBBETA
  • RAC-PK-beta
Protein TargetAkt2/PKBβ
Purification MethodNi2+/NTA-agarose & heparin-Sepharose™ chromatography
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P31751 # General protein kinase capable of phosphorylating several known proteins.
SIZE: 481 amino acids; 55769 Da
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DISEASE:SwissProt: P31751 # Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.
SIMILARITY: SwissProt: P31751 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight42.8kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of Crosstide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-339) and in bulk (cat# 14-339-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
14-339M 04053252332890

Related Products & Applications

Alternative Packsize

Catalog Number Description
14-339 Akt2/PKBβ (δPH, S474D) Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases