There is no minimal volume requirement. However, these devices can only concentrate down to approximately 50-100 microliters, so the starting volume should be chosen based on the desired degree of concentration.
UFC8010D
Amicon® Ultra-4 Centrifugal Filter Unit, registered for IVD use
Synonym(s):
Centrifugal ultrafiltration device, 10 kDa MWCO, 4 mL sample volume
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8 PKG
$87.60
24 PKG
$231.00
96 PKG
$790.00
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8 PKG
$87.60
24 PKG
$231.00
96 PKG
$790.00
About This Item
UNSPSC Code:
41104916
NACRES:
NB.24
Recommended Products
material
polyethylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pack of 24 ea (UFC801024D)
pack of 8 ea (UFC801008D)
pack of 96 ea (UFC801096D)
manufacturer/tradename
Amicon® Ultra
technique(s)
centrifugation: suitable (protein concentration and desalting)
protein extraction: suitable
protein purification: suitable
L
124 mm (4.88 in.)
diam.
17.3 mm
filtration area
3 cm2
volume
4 mL
working volume
≤4 mL
pore size
10 kDa MWCO
General description
This Amicon® Ultra 4 mL centrifugal device is registered for in vitro diagnostic use and intended for concentrating serum, urine, cerebrospinal fluid and other body fluids prior to analysis. It is comonly used in testing urine for Bence Jones protein to diagnose multiple myeloma. This device is also used for assessment of free hormones (i.e. thyroid hormones or testosterone) in serum.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
Application
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Customers Also Viewed
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What is the minimal volume to use in 4mL Amicon device?
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What is the shelf life and/or expiration date for Amicon devices?
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Non-IVD Amicon Devices
• There is no expiration date (post-2019) for non-IVD Amicon devices, but end users should check stock after 3 years.
• These devices fall under the standard one-year warranty from the date of receipt.
• The previous 3-year shelf life indication prior to 2019 no longer applies to non-IVD devices.IVD Amicon Devices.
• Specifically, 10 kDa MWCO only: Ultra-4 (UFC8010D) and Ultra-15 (UFC9010D).
• These devices have a 3-year Expiry Date designated on the label.
• The catalogue numbers end in "D".Helpful?
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