Amicon® Ultra-4 Centrifugal Filter Units
Provide both ultra-fast spin times and ultra-high recoveries
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Provide both ultra-fast spin times and ultra-high recoveries Less
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Overview
Specifications
Ordering Information
Documentation
References
| Reference overview | Application |
|---|---|
| Modification of Allergenicity and immunogenicity of formate dehydrogenase by conjugation with linear mono methoxy poly ethylene glycol: Improvement in detoxification of formate in methanol poisoning Muthuvel A., Rajamani R., Senthilvelan M., Manikandan s., Sheeladevi R. Clinica Chimica acta 374 (2006) 122-128 2006 | Protein Purification |
| Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis. Smejkal GB, Li C, Robinson MH, Lazarev AV, Lawrence NP, Chernokalskaya E. J Proteome Res. 2006 Apr;5(4):983-7. 2006 | Protein Concentration |
| Measurement of Pro-C-Type Natriuretic Peptide in Plasma Nielsen, S.J., Rehfeld, J.F., Pedersen, F., Kastrup, J., Videback, R., Goetze, J.P. Clinical Chemistry 51 (2005): 2173-2716 2005 | |
| Comparative Proteomic Analysis of Intra- and Interindividual Variation in Human Cerebrospinal Fluid Hu, Y., Malone, J.P., Fagan, A.M., Townsend, R.R., Holtzmann, D.M Molecular & Cellular Proteomics 4.12 (2005): 2000-2009 2005 | |
| Ultrafiltration for proteomic sample preparation E. Chernokalskaya, S. Gutierrez, A.M. Pit, J. T. Leonard Electrophoresis, 25: 2461-2468 2004 | Sample Prep |
FAQ
| Question | Answer | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amicon Ultra units are offered in what volumes? | Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes. | ||||||||||||
| What is the recommended rotor for Amicon Ultra units? | Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units. | ||||||||||||
| How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra? | Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest. | ||||||||||||
| Which membranes are available in Amicon Ultra units? | Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO. | ||||||||||||
| Can the Amicon Ultra be spun to dryness? | No, the Amicon Ultra has a built in deadstop. | ||||||||||||
| Can the Amicon Ultra be used with nucleic acids? | Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide. | ||||||||||||
| What is the maximum sample volume for Amicon Ultra? |
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| What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15? |
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| What is the chemical compatibility of Amicon Ultra? | Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support. | ||||||||||||
| Can Amicon Ultra units be sterilized with ethanol? | Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization. |
Related Products & Applications
Related Products By: Application Facete
| Protein Purification |
| Dialysis and Filtration |
Related Products By: Brand Facete
| Ultracel® |
| Amicon® |
Categories
| Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units |
- High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
- High retentate recovery of >90%
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes)
- Heat-sealed membrane minimizes downstream extractables
- 100% integrity tested for reliable performance
- Convenient sample monitoring with translucent housing and volume gradations
- Direct pipette sample access eliminates processing step to recover concentrate
- High concentration factors of 80–100X
Applications
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC
- Concentration of dilute or pre-purified proteins from column eluents
- Desalting, buffer exchange and protein dialysis
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Typical Spin Times to Filtrate Volume
)
Spin conditions: 4000 xg swinging bucket rotor at 25 °C, 4 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).
Typical Protein Recovery
| Membrane NMWL, kDa |
Protein Solute | Protein Retentate Volume, mL |
Protein Retentate Recovery, % |
| 3 | Cytochrome c, 12.4 kDa (0.25 mg/mL) |
0.19 | 98.7 |
| 10 | Cytochrome c, 12.4 kDa (0.25 mg/mL) |
0.20 | 95 |
| 30 | BSA, 67 kDa (1 mg/mL) |
0.20 | 95 |
| 50 | BSA, 67 kDa (1 mg/mL) |
0.08 | 94 |
| 100 | IgG, 156 kDa (1 mg/mL) |
0.34 | 91 |
| 4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min). | |||
| Volumes | |
| Maximum Initial Sample Volume, mL* | 4 mL |
| Typical Final Concentrate Volume | 50 µL |
| Centrifugal Force | |
| Materials | |
| Membrane | Ultracel regenerated cellulose |
| Device | Housing: Styrene/butadiene Tube: Polypropylene Cap and liner: Polyethylene |
| Dimensions | |
| Active Membrane Area | 3 cm² |
| Filtrate tube or vial (centrifuge tube) | |
| Diameter | 17.3 mm |
| Length (with cap and device) | 124 mm (4.88 in.) |
| Filter unit | |
| Diameter, mm | 17.2 |
| Length, mm | 73.4 |
| *Except for 23° fixed angle rotor: 3.5 mL | |