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NA02 Anti-2,2,7-Trimethylguanosine Mouse mAb (K121)

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) is validated for use in Affinity Purification, ICC, IP, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NA02
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
NA02-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
Catalogue NumberNA02
Brand Family Calbiochem®
Application Data
Detection of the 5' cap of snRNA by immunocytochemistry. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) (Cat. No. NA02) (0.8 μg/ml). Detection: fluorescence.
References
ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725.
Kramer, A., et al. 1984. Cell 38, 299.
Lerner, M.R., et al. 1980. Nature 283, 220.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlTransfer RNA (tRNA)
Positive controlHeterogenous nuclear RNA (hnRNA)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599. Paraffin Sections Volm, M., et al. 2000. Clin. Cancer Res. 6, 3236.
Key Applications Immunocytochemistry
Immunoprecipitation
Paraffin Sections
Application NotesAffinity Purification (use Cat. No. NA02A, see application references)
Immunocytochemistry (0.5-5 µg/ml)
Immunoprecipitation (1-2 µg, see application references)
Paraffin Sections (see application references)
Application CommentsAlso recognizes 7-methylguanosine-capped mRNA. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography (use Cat. No. NA02A). All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen2,2,7-trimethylguanosine
CloneK121
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
NA02-100UG 07790788053840

Documentation

Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Certificates of Analysis

TitleLot Number
NA02

References

Reference overview
Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725.
Kramer, A., et al. 1984. Cell 38, 299.
Lerner, M.R., et al. 1980. Nature 283, 220.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
ApplicationAffinity Purification (use Cat. No. NA02A, see application references)
Immunocytochemistry (0.5-5 µg/ml)
Immunoprecipitation (1-2 µg, see application references)
Paraffin Sections (see application references)
Application Data
Detection of the 5' cap of snRNA by immunocytochemistry. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) (Cat. No. NA02) (0.8 μg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
BackgroundThe U small nuclear ribonucleoprotein particles (snRNPs) are present in all eukaryotes studied thus far and are involved in the splicing of RNA molecules. snRNPs are composed of several proteins and one or two RNA molecules. The RNA molecules (snRNAs) contain a unique 5' terminal cap structure containing the nucleoside 2,2,7-trimethylguanosine. The 70 kDa protein component of U1 snRNP is cleaved during apoptosis, perhaps by a caspase. Monoclonal antibodies to the 5' cap structure react only to snRNAs.
HostMouse
Immunogen2,2,7-trimethylguanosine
CloneK121
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlHeterogenous nuclear RNA (hnRNA)
Negative controlTransfer RNA (tRNA)
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAlso recognizes 7-methylguanosine-capped mRNA. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography (use Cat. No. NA02A). All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725.
Kramer, A., et al. 1984. Cell 38, 299.
Lerner, M.R., et al. 1980. Nature 283, 220.
Application referencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599. Paraffin Sections Volm, M., et al. 2000. Clin. Cancer Res. 6, 3236.