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ABE2597-25UG Anti-AF9 (MLLT3)

Anti-AF9 (MLLT3), Cat. No. ABE2597, is a rabbit polyclonal antibody that detects AF-9 protein and is tested for use in ChIP-seq, Immunocytochemistry, and Western Blotting

ABE2597-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABE2597-25UG
DescriptionAnti-AF9 (MLLT3)
Alternate Names
  • Protein AF-9
  • ALL1-fused gene from chromosome 9 protein
  • Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein
  • MLLT3
  • YEATS domain-containing protein 3
Background InformationProtein AF-9 (UniProt: P42568; also known as ALL1-fused gene from chromosome 9 protein, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, MLLT3, YEATS domain-containing protein 3) is encoded by the MLLT3 (also known as AF9, YEATS3) gene (Gene ID: 4300) in human. AF9 is a chromatin reader component of the super elongation complex (SEC), which is shown to be essential to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. It specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. It recognizes and binds histone H3 crotonylated at lysine 9, and with slightly lower affinity histone H3 crotonylated at lysine 18. This binding is achieved through its YEATS domain (aa 8-112). It is also reported to recognize and bind histone H3 acetylated at lysine 9, but with lower affinity than crotonylated histone H3. Mutations of MLLT3 gene have been implicated in neurodevelopmental diseases, including epilepsy and ataxia. A chromosomal aberration involving MLLT3 has also been is associated with acute leukemias.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-AF9 (MLLT3), Cat. No. ABE2597, is a rabbit polyclonal antibody that detects AF-9 protein and is tested for use in ChIP-seq, Immunocytochemistry, and Western Blotting
Key Applications
  • ChIP-seq
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected AF9 (MLLT3) in NIH3T3 cell lysate.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected AF9 (MLLT3) in HeLa cells.

ChIP-seq Analysis: 20 mg (1u mg /ul) from a representative lot detected AF9 (MLLT3) in MOLM-13 cells expressing Flag-AF9 (Data courtesy of Drs. Liling Wan and Dr. C. David Allis, The Rockefeller University).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human AF9 (MLLT3) protein.
EpitopeC-terminus
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects AF9 (MLLT3) protein. It targets an epitope within 21 amino acids from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MLLT3
  • AF9
  • YEATs3
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~65 kDa observed; 63.35 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AF9 (MLLT3) in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE2597-25UG 04061841990338