Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

ABE1013-I Anti-ALKBH5 Antibody

Anti-ALKBH5 Antibody is an antibody against ALKBH5 for use in Western Blotting, Immunocytochemistry.

Anti-ALKBH5 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: ALKBH5, ABH5, OFOXD1 UniProt Number: Q6P6C2
ABE1013-I
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1013-I
DescriptionAnti-ALKBH5 Antibody
Alternate Names
  • RNA demethylase ALKBH5
  • Alkylated DNA repair protein alkB homolog 5
  • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5
Background InformationRNA demethylase ALKBH5 (UniProt Q6P6C2; also known as Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5) is encoded by the ALKBH5 (also known as ABH5, OFOXD1) gene (Gene ID 54890) in human. More than 100 types of postranscriptional RNA modifications are known, among which N6-methyladenosine (m6A) is the most common RNA modification. m6A modification mainly occurs in the consensus seqeunce motif RRm6ACH (R = A/G and H = A/C/U) located in stop codons, long internal exons as well as 3' UTR. RNA methyltransferases and demethylases are responsible for maintaining the level of cellular RNA m6A methylation. Two Fe2+ and 2-oxoglutarate (2OG) dependent AlkB dioxygenases family members, the fat mass and obesity-associated (FTO) enzyme and ALKBH5, catalyze the oxidative demethylation of abundant m6A residues. Both FTO and ALKBH5 are localized to nucleus and ALKBH5 gene knockdown is shown to affect cellular mRNA splicing in vitro and result in sterility phenotype in male mice in vivo.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ALKBH5 Antibody is an antibody against ALKBH5 for use in Western Blotting, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:200 dilution from a representative lot detected ALKBH5 in HeLa cells.
Biological Information
ImmunogenGST-tagged recombinant human ALKBH5 C-terminal fragment.
EpitopeC-terminal region.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with all three spliced isoforms of human ALKBH5 reported by UniProt (Q6P6C2).
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ALKBH5
  • ABH5
  • OFOXD1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~48 kDa observed. 52.26 kDa (isoform 1), 44.26 kDa (isoform 2), 43.05 kDa (isoform 3) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ALKBH5 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE1013-I 04055977332308