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MABN1866-25UL Anti-ANO6/TMEM16F Antibody, clone N429/19

Anti-ANO6/TMEM16F, clone N429/19, Cat. No. MABN1866, is a mouse monoclonal antibody that detects Anoctamin-6 (TMEM16F) and is tested for use in Immunohistochemistry (Paraffin).

MABN1866-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN1866-25UL
DescriptionAnti-ANO6/TMEM16F Antibody, clone N429/19
Alternate Names
  • Anoctamin-6
  • Small-conductance calcium-activated nonselective cation channel
  • SCAN channe
  • Transmembrane protein 16F
Background InformationAnoctamin-6 (UniProt: Q4KMQ2; also known as Small-conductance calcium-activated nonselective cation channel, SCAN channel, Transmembrane protein 16F, TMEM16F) is encoded by the ANO6 (also known as TMEM16F) gene (Gene ID: 196527) in human.TMEM16F is a multi-pass membrane glycoprotein that has 6 cytoplasmic domains, ten transmembrane domains, and four extracellular domains. It is expressed in embryonic stem cell, fetal liver, retina and in chronic myologenous leukemia and intestinal cancer. TMEM16F serves as a small-conductance calcium-activated nonselective cation channel that acts as a regulator of phospholipid scrambling in platelets and osteoblasts. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is shown to be essential to trigger the clotting system. In osteoblasts it is required for the deposition of hydroxyapatite during bone mineralization. It is also shown to generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes. Mutations in ANO6 gene have been linked to Scott syndrome, a mild bleeding disorder due to impaired surface exposure of procoagulant phosphatidylserine on platelets and other blood cells following activation with calcium elevating agents.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-ANO6/TMEM16F, clone N429/19, Cat. No. MABN1866, is a mouse monoclonal antibody that detects Anoctamin-6 (TMEM16F) and is tested for use in Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Biological Information
ImmunogenRecombinant fragment corresponding to the first 294 amino acids from the N-terminal, cytoplasmic domain of human Anoctamin-6 (ANO6).
Epitopecytoplasmic domain
CloneN429/19
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone N429/19 is a mouse monoclonal antibody that detects Anoctamin-6 (TMEM16F) . It targets an epitope within the N-terminal cytoplasmic domain.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ANO6
  • TMEM16F
Purification MethodProtein G purified
UniProt Number
Molecular Weight106.16 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected ANO6/TMEM16F in human kidney tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABN1866-25UL 04061842667642