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MABF3141-100UG Anti-ANTXR2/CMG-2 Antibody, clone 1H8

Anti-ANTXR2/CMG-2, clone 1H8, Cat. No. MABF3141, is a mouse monoclonal antibody that detects ANTXR2/CMG-2 and is tested for use in Western Blotting.

MABF3141-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3141-100UG
DescriptionAnti-ANTXR2/CMG-2 Antibody, clone 1H8
Alternate Names
  • Anthrax toxin receptor 2
  • Capillary morphogenesis gene 2vprotein
  • CMG-2
Background InformationAnthrax toxin receptor 2 (UniProt: P58335; also known as Capillary morphogenesis gene 2 protein, CMG-2) is encoded by the ANTRX2 (also known as CMG2) gene (Gene ID: 118429) in human. ANTRX2/CMG-2 is widely expressed, single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 34-318), a transmembrane domain (aa 319-341), and a cytoplasmic domain (aa 342-489). It shares about 58% amino acid identity with ANTXR1. Four isoforms of ANTRX2/CMG-2 have been described that are produced by alternative splicing. It is reported to be essential for cellular interactions with laminin and the extracellular matrix. It is an important regulator of collagen VI homeostasis. It mediates the transport of collagen to lysosomes for degradation. Its loss promotes accumulation of Collagen VI leading to nodule formation. Antrx2 -/- mice are shown to display massive accumulation of collagen VI in their uteri leading to fibrosis and sterility. It binds to collagen VI via its vWA domain (aa 44-213) in a MIDAS (metal ion-dependent binding domain) manner. (Ref.: Burgi, J., et al. (2017). Nat. Commun. 8; Article 15861; Chaudhary, A., et al. (2012). Cancer Cell. 21(2): 212-226).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-ANTXR2/CMG-2, clone 1H8, Cat. No. MABF3141, is a mouse monoclonal antibody that detects ANTXR2/CMG-2 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesTested applications

Western Blotting Analysis: A representative lot detected ANTXR2/CMG-2 in Western Blotting applications (Chaudhary, A., et al. (2012). Cancer Cell. 21(2):212-26; Szot, C., et al. (2018). J Clin Invest. 128(7):2927-2943).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenA recombinant fragment of 178 amino acids from the N-terminal half of human ANTXR2 with an N-terminal extension of GSHMLEDPRG.
EpitopeN-terminal half
Clone1H8
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1H8 is a mouse monoclonal antibody that detects Anthrax toxin receptor 2 (ANTXR2). It targets an epitope within the N-terminal half.
IsotypeIgG2cκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ANTXR2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~54 kDa observed; 53.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse lung tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ANTXR2/CMG-2 in Mouse lung tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF3141-100UG 04065270172532