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MABE163 Anti-ASF-1 (SF2) Antibody, clone AK96

Use Anti-ASF-1 (SF2) Antibody, clone AK96 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC to detect ASF-1 (SF2) also known as splicing factor arginine/serine-rich 1, Alternative-splicing factor 1 & pre-mRNA-splicing factor SF2 P33 subunit

Anti-ASF-1 (SF2) Antibody, clone AK96 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: SF2, ASF, ASF-1, SF2p33, SF2P33, SRp30a UniProt Number: Q07955
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MABE163
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE163
ReplacesMABE512
DescriptionAnti-ASF-1 (SF2) Antibody, clone AK96
Alternate Names
  • splicing factor, arginine/serine-rich 1
  • Alternative-splicing factor 1
  • pre-mRNA-splicing factor SF2, P33 subunit
  • splicing factor 22
  • ASF-1
  • SF2
Background InformationAlternative-splicing factor 1 (ASF-1), also known as SRp30a or SF2, is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.
References
Product Information
FormatPurified
Control
  • Human lymph node lysate
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-ASF-1 (SF2) Antibody, clone AK96 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC to detect ASF-1 (SF2) also known as splicing factor arginine/serine-rich 1, Alternative-splicing factor 1 & pre-mRNA-splicing factor SF2 P33 subunit
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ASF-1 (SF2) in human prostate carcinoma cells and human testis tissue.
Western Blotting Analysis: A representative lot was used by an independent laboratory in western blotting (Sinha, R., et al. (2010). Mol. Cell. Biol. doi:10.1128/MCB.01270-09).
Immunoprecipitation Analysis: A representative lot immunoprecipitated ASF-1 (SF2) in HeLa nuclear extracts (Jablonski, J. A., et al. (2010). Nucleic Acids Research. 38(4):1249-1260).
Biological Information
ImmunogenMBP-tagged recombinant protein corresponding to the N-terminus of human ASF-1 (SF2).
EpitopeN-terminus
CloneAK96
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes the N-terminus of ASF-1 (SF2).
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Immunogen sequence is identical in mouse and rat, so reactivity is predicted.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene Symbol
  • SF2
  • ASF
  • ASF-1
  • SF2p33
  • SF2P33
  • SRp30a
Purification MethodProtein G Purified
UniProt Number
Molecular Weight ~ 30 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human lymph node lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected ASF-1 (SF2) on 10 µg of human lymph node lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE163 04053252422515

Documentation

Anti-ASF-1 (SF2) Antibody, clone AK96 SDS

Title

Safety Data Sheet (SDS) 

Anti-ASF-1 (SF2) Antibody, clone AK96 Certificates of Analysis

TitleLot Number
Anti-ASF-1 (SF2), clone AK96 - 2428884 2428884
Anti-ASF-1 (SF2), clone AK96 - 2452469 2452469
Anti-ASF-1 (SF2), clone AK96 - 2003690 2003690
Anti-ASF-1 (SF2), clone AK96 - 2024893 2024893
Anti-ASF-1 (SF2), clone AK96 - 2154112 2154112
Anti-ASF-1 (SF2), clone AK96 - 2246823 2246823
Anti-ASF-1 (SF2), clone AK96 - 3284298 3284298
Anti-ASF-1 (SF2), clone AK96 - NG1881284 NG1881284
Anti-ASF-1 (SF2), clone AK96 - NRG1773119 NRG1773119
Anti-ASF-1 (SF2), clone AK96 -2858463 2858463
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References

Reference overviewPub Med ID
The transcriptional transactivator Tat selectively regulates viral splicing.
Jablonski, JA; Amelio, AL; Giacca, M; Caputi, M
Nucleic acids research  38  1249-60  2010

Show Abstract 		19966273 19966273