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MABC1092 Anti-ASPP2 (TP53BP2) Antibody, clone DX54.10

Anti-ASPP2 (TP53BP2), clone DX54.10, Cat. No. MABC1092, is a mouse monoclonal antibody that detects Apoptosis-stimulating of p53 protein 2 (ASPP2) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-ASPP2 (TP53BP2) Antibody, clone DX54.10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: TP53BP2, ASPP2, BBP UniProt Number: Q13625
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MABC1092
200 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IH(P), IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1092
DescriptionAnti-ASPP2 (TP53BP2) Antibody, clone DX54.10
Alternate Names
  • Apoptosis-stimulating of p53 protein 2
  • Bcl2-binding protein
  • Bbp
  • Renal carcinoma antigen NY-REN-51
  • Tumor suppressor p53-binding protein 2
  • 53BP2
  • p53-binding protein 2
  • p53BP2
Background InformationApoptosis-stimulating of p53 protein 2 (UniProt: Q13625; also known as Bcl2-binding protein, Bbp, Renal carcinoma antigen NY-REN-51, Tumor suppressor p53-binding protein 2, 53BP2, p53-binding protein 2, p53BP2) is encoded by the TP53BP2 (also known as ASPP2, BBP) gene (Gene ID: 7159) in human. 53BP2 plays a central role in regulation of apoptosis and cell growth. It is widely expressed and its expression is observed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte 53BP2 is induced following DNA damage by UV radiation. It is down-regulated by wild-type p53/TP53, but not by mutant p53/TP53. It regulates p53/TP53 action by enhancing the DNA binding and transactivation function of TP53 on the promoters of pro-apoptotic genes in vivo. 53BP2 is shown to inhibit the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. 53BP2 contains four ankyrin repeats and one SH3 domain are required for a specific interactions with TP53. The 53BP2 binding site on the p53 core domain consists of evolutionarily conserved regions that are frequently mutated in human cancers. Lower level of 53BP2 expression is observed in breast carcinomas expressing a wild-type TP53 protein.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ASPP2 (TP53BP2), clone DX54.10, Cat. No. MABC1092, is a mouse monoclonal antibody that detects Apoptosis-stimulating of p53 protein 2 (ASPP2) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation Analysis: A representative lot detected ASPP2 (TP53BP2) in Immunoprecipitation applications (Samuels-Lev, Y., et. al. (2001). Mol Cell. 8(4):781-94).

Immunohistochemistry Analysis: A representative lot detected ASPP2 (TP53BP2) in Immunohistochemistry applications (Wang, Y., et. al. (2014). Nat Cell Biol. 16(11):1092-104).

Immunocytochemistry Analysis: A representative lot detected ASPP2 (TP53BP2) in Immunocytohemistry applications (Wang, Y., et. al. (2013). Cell Death Differ. 20(4):525-34; Wang, Y., et. al. (2014). Nat Cell Biol. 16(11):1092-104).

Western Blotting Analysis: A representative lot detected ASPP2 (TP53BP2) in Western Blotting applications (Wang, Y., et. al. (2013). Cell Death Differ. 20(4):525-34; Samuels-Lev, Y., et. al. (2001). Mol Cell. 8(4):781-94).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 438 amino acids from the C-terminal half of human Apoptosis-stimulating of p53 protein 2 (TP53BP2).
EpitopeC-terminus
CloneDX54.10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone DX54.10 detects Apotosis -stimulating of p53 protein 2 (TP53BP2) in human cells. It targets an epitope with in 438 amino acids from the C-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • TP53BP2
  • ASPP2
  • BBP
Purification MethodProtein G purified
UniProt Number
Molecular Weight~170 kDa observed; 125.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in U2OS-iASPP and U2OS RISC-free cell lysates.

Western Blotting Analysis: A 1:125 dilution of this antibody detected ASPP2 in lysates of U2OS cells transfected with siRNA to knock down iASPP and in RISC-free U2OS cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC1092 04054839322464