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MABE1933-25UG Anti-ASXL1 Antibody, clone 12F9

Anti-ASXL1, clone 12F9, Cat. No. MABE1933, is a rat monoclonal antibody that detects ASXL1 and is tested for use in Immunocytochemistry and Western Blotting.

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MABE1933-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABE1933-25UG
DescriptionAnti-ASXL1 Antibody, clone 12F9
Alternate Names
  • Polycomb group protein ASXL1
  • Additional sex combs-like protein 1
Background InformationPolycomb group protein ASXL1 (UniProt: Q8IXJ9; also known as Additional sex combs-like protein 1) is encoded by the ASXL1 (also known as KIAA0978) gene (Gene ID: 171023) in human. ASXL1 is a member of the ASXL family that is expressed at low levels in various tissues. However, higher expression is reported in testes. It is a member of the ASXL family with a highly conserved ASX homology (ASXH) domain at the N-terminal region and a plant homeodomain (PHD) finger (aa 1503-1540) at the C-terminal region. The ASXH domain mediates its interaction with a partner protein BAP1, which is an essential component of the polycomb repressive deubiquinase complex (PR-DUB), in which it is involved in deubiquitination of monoubiquitinated histone H2A at lysine 119 (H2AK119ub). ASXL1 also acts as a sensor of N6-methyladenosine methylation on DNA (m6A). It recognizes and binds m6A DNA, leading to its ubiquitination and degradation by TRIP12, thereby inactivating the PR-DUB complex and regulating polycomb silencing. Its HARE-HTH-type domain (aa 11-86) recognizes and binds m6A. Mutations in ASXL1 gene are known to perturb development and cause Bohring-Opitz syndrome that is characterized by severe intrauterine growth retardation, poor feeding, and mental retardation. Some mutations are also observed in myeloid neoplasms, including myelodysplastic syndromes, chronic myelomonocytic leukemia, and acute myeloid leukemia and are associated with poor prognosis. (Ref.: Matheus, F., et al. (2019). Stem Cell Reports 12(5); 861-868; Asada, S., et al. (2018). Nat. Commun. 9: Article 2733; Scheuermann, JC., et al. (2010). Nature. 465(7295); 243-247).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-ASXL1, clone 12F9, Cat. No. MABE1933, is a rat monoclonal antibody that detects ASXL1 and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesTested Applications

Immunocytochemistry Analysis: A representative lot detected ASXL1 in Immunocytochemistry applications (Matheus, F.. et al. (2019). Stem Cell Reports. 12(5):861-868).

Western Blotting Analysis: A representative lot detected ASXL1 in Western Blotting applications (Matheus, F.. et al. (2019). Stem Cell Reports. 12(5):861-868).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to the first 618 amino acids from the N-terminal region of human Polycomb group protein ASXL1.
EpitopeN-terminal
Clone12F9
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 12F9 is a rat monoclonal antibody that detects human Polycomb group protein ASXL1.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ASXL1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~165 kDa observed; 165.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ASXL1 in Huma spleen tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1933-25UG 04065267327624