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AB2953 Anti-ATG3 Antibody

Anti-ATG3 Antibody detects level of ATG3 & has been published & validated for use in WB.

Anti-ATG3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: ATG3, PC3-96, APG3-LIKE, APG3, hApg3, APG3L, APG3-like UniProt Number: Q9NT62
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AB2953
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB2953
DescriptionAnti-ATG3 Antibody
Alternate Names
  • ATG3 autophagy related 3 homolog (S. cerevisiae)
  • APG3 autophagy 3-like (S. cerevisiae)
  • autophagy-related protein 3
  • autophagy Apg3p/Aut1p-like
  • APG3 autophagy 3-like
  • Protein PC3-96
Background InformationATG3 is thought to be an autophagy-related ubiquitin carrier protein (E2)-like enzyme that is the go between for the conjugation on ATG8 and phosphatidylethanolamine (PE). This compound is necessary for autophagy, which is a process in which a cell is degraded by its own lysosomal process.
References
Product Information
FormatAffinity Purified
Control
  • HL-60 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ATG3 Antibody detects level of ATG3 & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human ATG3.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with human.
Other homologies: Ape, rhesus macaque, equine, porcine, and bovine (99% sequence homology). Mouse (98% sequence homology). Rat (97% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryAutophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM].
Gene Symbol
  • ATG3
  • PC3-96
  • APG3-LIKE
  • APG3
  • hApg3
  • APG3L
  • APG3-like
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.

SUBUNIT STRUCTURE: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.

SUBCELLULAR LOCATION: Cytoplasm

TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.

PTM: Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy (By similarity).

SEQUENCE SIMILARITIES: Belongs to the ATG3 family.

SEQUENCE CAUTION: The sequence BAB15237.1 differs from that shown. Reason: Erroneous initiation.

Isoform 2: The sequence AAH02830.1 differs from that shown. Reason: Frameshift at position 311.
Molecular Weight~37 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HL-60 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected ATG3 in 10 µg of HL-60 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB2953 04053252482717