Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABE1991-25UG Anti-AUF1 Antibody, clone 5B9

Anti-AUF1, clone 5B9, Cat. No. MABE1991, is a mouse monoclonal antibody that detects AU-rich element RNA-binding protein 1 (AUF1) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

View Products on Sigmaaldrich.com
MABE1991-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABE1991-25UG
DescriptionAnti-AUF1 Antibody, clone 5B9
Alternate Names
  • Heterogeneous nuclear ribonucleoprotein D0
  • hnRNP D0
  • AU-rich element RNA-binding protein 1
Background InformationHeterogeneous nuclear ribonucleoprotein D0 (UniProt: Q14103; also known as hnRNP D0, AU-rich element RNA-binding protein 1, AUF1) is encoded by the HNRNPD (also known as AUF1, HNRPD) gene (Gene ID: 3184) in human. AUF1 is one of several proteins that exhibits high-affinity binding and specificity for AU-rich elements (AREs) in 3 untranslated regions (3 UTRs). It also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. AUF1 is also considered as an RNA destabilizing protein. Four isoforms of AUF1 have been described that are produced by alternative splicing. Although the role of each isoform has not been fully characterized, a direct correlation has been observed between each AUF1 isoform s binding affinity and its RNA destabilizing activity toward different AREs, with the isoforms p37 and p42 being the most effective. It contains several RNA binding domains and each of these specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat in a weaker manner. AUF1 is reported to be localized in cytoplasmic mRNP granules containing untranslated mRNAs and its cytoplasmic localization oscillates diurnally. AUF1 is also shown to a bcl2 mRNA binding protein and all four isoforms can form complexes with bcl2 ARE. AUF1 is also involved in the downregulation of ARE mediated bcl2 mRNA during apoptosis. Clone 5B9 is shown to bind to an epitope within the RNA binding domain II (RBD2). This is a common epitope present in RBD2 of all isoforms of AUF1. (Ref.: Dogar, MA., et al. (2018). PLoS One. 13(11); e0206823; Kajita, Y., et al. (1995). J. Biol. Chem. 270(38); 22167-22175; Ishikawa, F., et al. (1993). Mol. Cell Biol. 13(7); 4301-4310).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-AUF1, clone 5B9, Cat. No. MABE1991, is a mouse monoclonal antibody that detects AU-rich element RNA-binding protein 1 (AUF1) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesTested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected AUF1 in human testis tissue sections.

Western Blotting Analysis: A representative lot detected AUF1 in Western Blotting applications (Gouble, A., et al. (2002). Cancer Res. 62(5):1489-95).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AUF1 in Human stomach tissue lysates.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHuman AUF1
EpitopeInternal
Clone5B9
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5B9 is a mouse monoclonal antibody that detects human AU-rich element RNA-binding protein 1 (AUF1). It targets an epitope within the RBD2 domain from the internal region.
IsotypeIgM
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • HNRNPD
Purification MethodLigatrap
UniProt Number
Molecular Weight~40 kDa observed; 38.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human stomach tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected AUF1 in human stomach tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1991-25UG 04065267613970