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MABE1829-25UG Anti-Acetyl β-Catenin (Lys49) Antibody, clone mAb-K49-Ac

Anti-Acetyl -Catenin (Lys49), clone mAb-K49-Ac, Cat. No. MABE1829, is a rat monoclonal antibody that targets Catenin -1 acetylated on Lysine 49 and is tested in Dot Blot, Immunoprecipitation, and Western Blotting.

MABE1829-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABE1829-25UG
DescriptionAnti-Acetyl β-Catenin (Lys49) Antibody, clone mAb-K49-Ac
Alternate Names
  • Catenin beta-1
Background InformationCatenin beta-1 (UniProt: P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, OK/SW-cl.35, PRO2286) gene (Gene ID: 1499) in human. Catenin beta-1 is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3 beta that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome. In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes. O-glycosylation at serine 23 is reported to decreases its nuclear localization and transcriptional activity. Wnt/beta-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation. In ESC beta-catenin is trimethylated on lysine 49 by Ezh2 methyltransferase and acetylated by CBP. The trimethylated form represses neuronal differentiation genes Sox1 and Sox3. The K49 acetylated form acts as a transcriptional co-activator of the key mesodermal differentiation gene t-brachyury (t-bra). K49A mutant form of beta-catenin is shown cause defects in ESC differentiation. Mutations in CTNNB1 gene are shown to cause colorectal cancers, medulloblastoma, and ovarian cancers. (Ref.: Hoffmeyer, K., et al. (2017). Cell Reports 18(12); 2815-2824; Levy, L., et al. (2004). Mol. Cell Biol. 24(8); 3404-3414).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Acetyl -Catenin (Lys49), clone mAb-K49-Ac, Cat. No. MABE1829, is a rat monoclonal antibody that targets Catenin -1 acetylated on Lysine 49 and is tested in Dot Blot, Immunoprecipitation, and Western Blotting.
Key Applications
  • Dot Blot
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Acetyl a-Catenin (Lys49) in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

Dot Blot Analysis: A representative lot detected Acetyl -Catenin (Lys49) in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Acetyl -Catenin (Lys49) in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Biological Information
ImmunogenBSA-conjugated linear peptide corresponding to 19 amino acids surrounding acetylated lysine 49 from human -catenin.
ClonemAb-K49-Ac
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone mAb-K49-Ac is a rat monoclonal antibody that detects -Catenin acetylated on lysine 49.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Canine based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CTNNB1
  • CTNNB
  • OK/SW-cl.35
  • PRO2286
Modifications
  • Acetylation
Purification MethodProtein G purified
UniProt Number
Molecular Weight~90 kDa observed; 85.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SW 480 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Acetylated -Catenin (Lys49) in SW 480 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1829-25UG 04061841440697

Documentation

Anti-Acetyl β-Catenin (Lys49) Antibody, clone mAb-K49-Ac SDS

Title

Safety Data Sheet (SDS) 

Anti-Acetyl β-Catenin (Lys49) Antibody, clone mAb-K49-Ac Certificates of Analysis

TitleLot Number
Anti-Acetyl β-Catenin (Lys49), clone mAb-K49-Ac - Q3416339 Q3416339

References

Reference overviewPub Med ID
Trimethylation and Acetylation of β-Catenin at Lysine 49 Represent Key Elements in ESC Pluripotency.
Hoffmeyer, K; Junghans, D; Kanzler, B; Kemler, R
Cell Rep  18  2815-2824  2017

Show Abstract 		28329675 28329675