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MAB1581-I-25UG Anti-Aggrecan Core Protein Antibody, clone Cat-315

Anti-Aggrecan Core Protein, clone Cat-315, Cat. No. MAB1581-I, is a mouse monoclonal antibody that detects Aggrecan Core Protein and is used in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

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MAB1581-I-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMAB1581-I-25UG
DescriptionAnti-Aggrecan Core Protein Antibody, clone Cat-315
Alternate Names
  • Anti-Chondroitin Sulfate Proteoglycan core protein
  • CSPG1
  • Cat-315
Background InformationAggrecan Core Protein (UniProt: A0A5F5XE37; also known as Anti-Chondroitin Sulfate Proteoglycan core protein, CSPG1) is encoded by the ACAN gene (Gene ID: 101084542) in cat. Chondroitin sulfate proteoglycans (CSPGs) are a family of large extracellular matrix molecules, composed of a central core protein to which a varying number of glycosaminoglycan (GAG) side chains are attached. They are widely expressed in the mammalian central nervous system. CSPG1 consists of a protein core and a chondroitin sulfate side chain. CSPG1 lacks a transmembrane domain and the entire molecule is extracellular and, therefore, is considered a constituent of brain extracellular matrix. It is synthesized with a signal peptide (aa 1-16, which is subsequently cleaved off to generate the mature form. CSPG1 plays a role in neural development, plasticity, and post-injury response. CSPGs are involved in cell adhesion, cell growth, receptor binding, and cell migration. They are reported to inhibit axon regeneration after spinal cord injury and contribute to glial scar formation, which blocks new axons from growing into the injury site. During development CSPGs are reported to act as guidance cues for developing growth cones. In the adult, they stabilize synaptic connections and prevent aberrant axonal sprouting. Clone Cat-315 is shown to recognize a chondroitin sulfate proteoglycan (CSPG) expressed on the surface of subsets of neurons in many areas of the mammalian CNS. (Ref.: Siebert, JR., and Osterhout, DJ. (2011). J. Neurochem. 119(1); 186-188; Mathews, RT., et al. (2002). J. Neurosci. 22(17); 7536-7547; Lander, C., et al. (1998). J. Neurosci. 18(1); 174-183).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-Aggrecan Core Protein, clone Cat-315, Cat. No. MAB1581-I, is a mouse monoclonal antibody that detects Aggrecan Core Protein and is used in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesTested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Aggrecan Core Protein in cortex tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:150 dilution from a representative lot detected Aggrecan Core Protein in rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Aggrecan Core Protein in Rat e18 cortical cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Aggrecan Core Protein in Immunoprecipitation applications (Lander, C., et. al. (1998). J Neurosci. 18(1):174-83).

Immunohistochemistry Applications: A representative lot detected Aggrecan Core Protein in Immunohistochemistry applications (Grant, E., et. al. (2016). Cereb Cortex. 26(3):1336-1348; Lander, C., et. al. (1998). J Neurosci. 18(1):174-83; Quattromani, M.J., et. al. (2018). Mol Neurobiol. 55(3):2196-2213).

Immunofluorescence Analysis: A representative lot detected Aggrecan Core Protein in Immunofluorescence applications (Quattromani, M.J., et. al. (2018). Mol Neurobiol. 55(3):2196-2213).

Western Blotting Analysis: A representative lot detected Aggrecan Core Protein in Western Blotting applications (Choi, B.R., et. al. (2020). Cell Rep. 31(5):107540; Quattromani, M.J., et. al. (2018). Mol Neurobiol. 55(3):2196-2213; Lander, C., et. al. (1998). J Neurosci. 18(1):174-83).

Immunocytochemistry Analysis: A representative lot detected Aggrecan Core Protein in Immunocytochemistry applications (Lander, C., et. al. (1998). J Neurosci. 18(1):174-83).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFeline brain proteoglycans.
EpitopeExtracellular domain
CloneCat-315
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone Cat-315 is a mouse monoclonal antibody that detects Aggrecan core protein (CSPG1). It detects an epitope on the extracellular region.
IsotypeIgM
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Feline based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • ACAN
Purification MethodLigatrap
UniProt Number
Molecular Weight~260 kDa observed; 235.2 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Aggrecan Core Protein in Rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB1581-I-25UG 04065266553789