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16-293 Sigma-AldrichAnti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 488

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor 488 is an antibody against Akt/PKB for use in FC, IC, IF & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: AKT1, RAC, PRKBA, MGC99656, RAC-ALPHA, RAC-PK-alpha, C-AKT, PKB, AKT UniProt Number: P31749

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	FC, ICC, IF, WB	M	AlexaFluor®488	Monoclonal Antibody

Description
Catalogue Number	16-293
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 488
Alternate Names	PKB PKD-alpha AKT1 Protein Kinase B

References

Product Information
Format	AlexaFluor®488
Quality Level	MQ100

Applications
Application	Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor 488 is an antibody against Akt/PKB for use in FC, IC, IF & WB.
Key Applications	Flow Cytometry Immunocytochemistry Immunofluorescence Western Blotting

Biological Information
Immunogen	GST-fusion protein corresponding to residues 1-149 of human Akt1. Clone SKB1.
Clone	clone SKB1
Host	Mouse
Specificity	Recognizes Akt1/PKBα > Akt2/PKBβ (30%) > Akt3/PKBγ (weak to none) based on immunoblot analysis using 100ng recombinant fusion protein for each isoform.
Isotype	IgG
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001014431.1 NM_001014432.1 NM_005163.2
Entrez Gene Summary	The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Gene Symbol	AKT1 RAC PRKBA MGC99656 RAC-ALPHA RAC-PK-alpha C-AKT PKB AKT
Purification Method	Protein G Purified
UniProt Number	P31749
UniProt Summary	FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. SIZE: 480 amino acids; 55686 Da SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). TISSUE SPECIFICITY: In all human cell types so far analyzed. DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane. PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A). SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight	60 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Immunocytochemistry and Flow Cytometry
Usage Statement	Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Immunocytochemistry and Flow Cytometry

Usage Statement

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	1 year at 4°C from date of shipment

Packaging Information
Material Size	50 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
16-293	04053252273049

Documentation

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 488 Certificates of Analysis

Title	Lot Number
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor#174; 488 conjugate - 1994386	1994386
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor#174; 488 conjugate - 2145963	2145963
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor#174; 488 conjugate - JBC1854164	JBC1854164
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluorreg; 488 conjugate - D6D024	D6D024
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate (mouse monoclonal IgG)	3158056
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate (mouse monoclonal IgG)	3142394
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate - 3026849	3026849
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate -2532560	2532560
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate -2538632	2538632
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate -2729446	2729446

Related Products & Applications

Related Products

Catalog Number	Description
04-736	Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal
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05-1003	Anti-phospho-Akt (Ser473) Antibody, clone 6F5
05-591	Anti-Akt/PKB Antibody, PH Domain, clone SKB1
05-669	Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6
05-736	Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal
05-796	Anti-Akt1/PKBα Antibody, clone AW24, rabbit monoclonal
05-802R	Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal
07-416	Anti-Akt1/PKBα Antibody
07-789	Anti-phospho-AKT (Thr34) Antibody

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