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MABN43 Anti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4

This Anti--Apolipoprotein E4 antibody is validated for use in IP, WB, IH for the detection of ApoE4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: APOE UniProt Number: P02649
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MABN43
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN43
DescriptionAnti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4
Alternate Names
  • Apolipoprotein E
  • Apo-E
  • ApoE4
  • Apolipoprotein E4
Background InformationApolipoprotein is a polymorphic plasma protein that exists as three isoforms: ApoE4, ApoE3, and ApoE2, which differ in the combination of cysteine and arginine residues located at position 122 and 158. The 35 kDa ApoE4 isoform contains arginine at both locations, and is produced in the liver and brain, although it is widely expressed in other tissues, such as the lung, spleen, and ovary. Like other isoforms, ApoE4 is a ligand for low density lipoprotein receptors (LDL) and plays a critical role in the transport and metabolism of lipids. In addition to its role in maintaining cardiovascular function, ApoE4 is also critically involved in neuronal plasticity, synaptogenesis, aging, and neurodegenerative disorders. Mice deficient in ApoE4 show age-dependent abnormalities in synaptic structure and impaired long term potentiation. Additionally, ApoE4 has a well-documented role in the pathology of Alzheimer’s disease.
References
Product Information
FormatPurified
Control
  • Recombinant Apolipoprotein E protein
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti--Apolipoprotein E4 antibody is validated for use in IP, WB, IH for the detection of ApoE4.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A 1:2,000-4,000 dilution from a representative lot detected ApoE4 in human Alzheimer's diseased brain and human cerebellum tissues.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect ApoE4 in CHO cells secreting ApoE4. (Image courtesy of Dr. Egon Ogris, Medical University of Vienna)
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human ApoE4.
Clone4E4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes ApoE4 but not ApoE3 or ApoE2.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APOE
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~35 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in recombinant ApoE4 protein.

Western Blot Analysis: A 1:100,000 dilution detected ApoE4 in 10 µg of recombinant ApoE4 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABN43 04053252695889

Documentation

Anti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4 SDS

Title

Safety Data Sheet (SDS) 

Anti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4 Certificates of Analysis

TitleLot Number
Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 2464648 2464648
Anti-Apolipoprotein E4 (ApoE4), -2604838 2604838
Anti-Apolipoprotein E4 (ApoE4), -2820249 2820249
Anti-Apolipoprotein E4 (ApoE4), clone 4E4 2478024
Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3231009 3231009
Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3290432 3290432
Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3445791 3445791
Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3515609 3515609
Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3708265 3708265
Anti-Apolipoprotein E4 (ApoE4), clone 4E4 - 3957440 3957440
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