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ABE70-I-25UG Anti-BAF180/PBRM1

Anti-BAF180-PBRM1, Cat. No. ABE70-I, is a rabbit polyclonal antibody that detects BAF180/PBRM1 and is tested for use in Western Blotting.

ABE70-I-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABE70-I-25UG
DescriptionAnti-BAF180/PBRM1
Alternate Names
  • Protein polybromo-1
  • hPB1
  • BRG1-associated factor 180
  • Polybromo-1D
Background InformationProtein polybromo-1 (UniProt: Q86U86; also known as hPB1, BRG1-associated factor 180, BAF180, Polybromo-1D) is encoded by the PBRM1 (also known as BAF180, PB1) gene (Gene ID: 55193) in human. BAF180 is a widely expressed, bromodomain containing nuclear protein that is an essential component of the SWI/SNF-B (PBAF) chromatin remodeling complex. It is involved in transcriptional activation and repression of select genes by chromatin remodeling. It contains six bromodomains, two bromo-adjacent homology (BAH) domains (aa 956-1074 and 1156-1272), and one HMG-box (aa 1379-1447). The bromodomains recognize and bind to acetylated lysines and BAH domains mediate protein-protein interaction. Its HMG-box domain binds to DNA. BAF180 acts as a negative regulator of cell proliferation. It plays a role in DNA repair mechanisms and acts as a tumor suppressor. It is also involved in silencing transcription near double-stranded DNA breaks (DSB). The BAH domains of BAF180 are shown to be essential for ubiquitination of PCNA. BAF180 depleted cells can not produce proper levels of ubiquitinated PCNA that leads to the inability to replicate past DNA lesions. Mutations in PBRM1 gene have been linked to high incidence of renal cell carcinoma. Nine isoforms of BAF180 have been described that are produced by alternative splicing. (Ref.: Hopson, S., and Thompson, MJ. (2017). ACS Chem. Biol. 12(10); 2482-2490; Varela, I., et al. (2011). Nature. 469(7331); 539-542).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ200
Applications
ApplicationAnti-BAF180-PBRM1, Cat. No. ABE70-I, is a rabbit polyclonal antibody that detects BAF180/PBRM1 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesNote: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 740 amino acids from the C-terminal half of human BAF180
EpitopeC-terminal half
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Protein polybromo-1 (BAF180). It targets an epitope within the C-terminal half.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with non-Human primates.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PBRM1
Purification MethodProtein A purified
UniProt Number
Molecular Weight~200 kDa observed; 192.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Protein polybromo-1 (BAF180) in Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE70-I-25UG 04065269135265