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MABE1083 Anti-BAP1 Antibody, clone 2A2

Anti-BAP1 Antibody, clone 2A2, Cat. No. MABE1083, is a highly specific mouse monoclonal antibody that targets BAP1 and has been tested in Western Blotting.

Anti-BAP1 Antibody, clone 2A2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: BAP1, KIAA0272, hucep-6 UniProt Number: Q92560
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MABE1083
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1083
DescriptionAnti-BAP1 Antibody, clone 2A2
Alternate Names
  • Ubiquitin carboxyl-terminal hydrolase BAP1
  • BRCA1-associated protein 1
  • Cerebral protein 6
Background InformationUbiquitin carboxyl-terminal hydrolase BAP1 (EC 3.4.19.12; UniProt Q92560; also known as BRCA1-associated protein 1, Cerebral protein 6) is encoded by the BAP1 (also known as KIAA0272, hucep-6) gene (Gene ID 8314) in human. Originally identified and named as a BRCA1-interacting protein, BAP1 is a tumor suppressor that participates in DNA-damage response, DNA synthesis and cell cycle progression. BAP1 belongs to the ubiquitin C-terminal hydrolase (UCH) family of deubiquitin enzymes (DUBs), all members of which, including UCH-L1, UCH-L3 and UCH-L5, are characterized by a conserved UCH catalytic domain (CD; BAP1 a.a. 1-235). BAP1 and UCH-L5 also share a conserved C-terminal ULD domain (a.a. 640-710 of BAP1). The region between BAP1 CD and ULD is important for binding to the HCF-1 protein. In addition, BAP1 contains a C-terminal extension (CTE; a.a. 711-729) following its ULD. Study shows that while CTE tethers BAP1 to the nucleosome core, additional interaction with ASXL1 DEUBiquitinase ADaptor domain (DEUBAD) is needed to allow BAP1 substrate processing by increasing it's affinity towards ubiquitin. In a similar fashion, UCH-L5 activity is reugulated via interaction with DEUBAD of RPN13 and INO80G. BAP1 is implicated in transcription regulation due to its interaction with transcription-related proteins, such as FOXK2, HCF-1, OGT, LSD2, MBD-6, MBD-5, ASXL1 and ASXL2. BAP1 loss leads to a variety of cancers, including metastatic cutaneous and uveal melanoma, pleural mesothelioma, renal cell carcinoma and bladder tumours.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-BAP1 Antibody, clone 2A2, Cat. No. MABE1083, is a highly specific mouse monoclonal antibody that targets BAP1 and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenRecombinant full-length human BAP1.
Clone2A2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2A2 targets human BAP1 UCH domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BAP1
  • KIAA0272
  • hucep-6
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~80 kDa observed. 80.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in transfected HEK293 cells.

Western Blotting Analysis: 2 µg/mL of this antibody detected exogenously expressed human BAP1 in 10 µg lysate from transfected HEK293 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1083 04054839088605