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MABE443 Anti-BPTF Antibody, clone 15B1.1

This Anti-BPTF Antibody, clone 15B1.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of BPTF .

Anti-BPTF Antibody, clone 15B1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: Bptf UniProt Number: E9Q6A7
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MABE443
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE443
DescriptionAnti-BPTF Antibody, clone 15B1.1
Alternate Names
  • Protein Bptf
  • BPTF
Background InformationNucleosome-remodeling factor (NURF) subunit BPTF (UniProt E9Q6A7 & A2A654; also known as Bromodomain and PHD finger-containing transcription factor, Fetal Alzheimer antigen) is encoded by the Bptf (also known as 9430093H17Rik, Falz) gene (Gene ID 207165) in murine species. ATP-dependent chromatin remodeling complexes are classified into four major subfamilies (SWI/SNF, ISWI, CHD, and INO80). NURF is the founding member of ISWI remodeling complexes. Mammalian NURF is composed of BPTF, the ATPase SNF2L, and the Trp-Asp (WD) repeat protein pRBAP46/48, with BPTF being the largest subunit among the three. BPTF PHD finger-mediated binding of H3K4me3 stabilizes the NURF complex on chromatin, resulting in NURF-catalyzed nucleosome sliding. The NURF remodeling complex is essential for mammalian development and executes epigenetic regulation in multiple signaling pathways, including heat shock, TGF-beta/SMAD, JAK/STAT, WNT/beta-catenin, RB, and nuclear hormone receptors.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-BPTF Antibody, clone 15B1.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of BPTF .
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected BPTF in human testis, rat colon, and mouse colon tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to mouse BPTF.
Clone15B1.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Bptf
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~322 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected BPTF in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE443 04055977175356

Documentation

Anti-BPTF Antibody, clone 15B1.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-BPTF Antibody, clone 15B1.1 Certificates of Analysis

TitleLot Number
Anti-BPTF, clone 15B1.1 - 3303094 3303094
Anti-BPTF, clone 15B1.1 - 4126643 4126643
Anti-BPTF, clone 15B1.1 -Q2578156 Q2578156
Anti-BPTF, clone 15B1.1 Monoclonal Antibody 3153207

Technical Info

Title
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White Paper: Further considerations of antibody validation and usage.