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MABE60 Anti-BRG1 Antibody, clone 3G4

Anti-BRG1 Antibody, clone 3G4 is a rat monoclonal Antibody for detection of BRG1, also known as BRG1-associated factor 190A, SNF2-like 4, and SNF2-BETA, and has been validated in Immunocytochemistry and Western Blotting applications.

Anti-BRG1 Antibody, clone 3G4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology, Transcription Factors Gene Symbol: SMARCA4, BAF190A, BRG1, MRD16, RTPS2, SNF2B, SNF2L4 UniProt Number: P51532
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MABE60
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICC, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE60
DescriptionAnti-BRG1 Antibody, clone 3G4
Alternate Names
  • Transcription activator BRG1
  • ATP-dependent helicase SMARCA4
  • BAF190A
  • BRG1-associated factor 190A
  • hSNF2b
  • Mitotic growth and transcription activator
  • Protein brahma homolog 1
  • Protein BRG-1
  • SNF2-beta
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
Background InformationTranscription activator BRG1 (UniProt P51532; also known as ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, hSNF2b, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4) is encoded by the SMARCA4 (also known as BAF190A, BRG1, MRD16, RTPS2, SNF2B, SNF2L4) gene (Gene ID 6597) in human. The SWI/SNF-like BRG1/BRM-associated factor (BAF) chromatin-remodeling complexes drive conformational changes of nucleosomes in an ATP-dependent manner and play important roles in cell-cycle progression, DNA repair, and development. The SWI/SNF complexes contain 10-12 interchangeable subunits, with either BRG1/SMARCA4 or BRM/SMARCA2 as the ATPase subunit. Brg1 is also shown to maintain Polycomb-mediated repression of non-mesodermal developmental regulators. Brg1-knockout leads to disruption of murine ESC cardiomyocyte differentiation and dysregulation of lineage-specific gene expression during mesoderm induction.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-BRG1 Antibody, clone 3G4 is a rat monoclonal Antibody for detection of BRG1, also known as BRG1-associated factor 190A, SNF2-like 4, and SNF2-BETA, and has been validated in Immunocytochemistry and Western Blotting applications.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected BRG1 (SMARCA4) in THP-1 human leukemia monocytic cell lysates (Cruickshank, V.A., et al. (2015). PLoS One. 10(11):e0142806).

Immunocytochemistry Analysis: A representative lot detected nuclear euchromatin BRG1 immunoreactivity in C2C12 murine myoblasts (Ohkawa, Y., et al. (2009). Hybridoma (Larchmt). 28(6):463-466).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human BRG1.
Clone3G4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SMARCA4
  • BAF190A
  • BRG1
  • MRD16
  • RTPS2
  • SNF2B
  • SNF2L4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~185 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected BRG1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE60 04053252747021

Documentation

Anti-BRG1 Antibody, clone 3G4 SDS

Title

Safety Data Sheet (SDS) 

Anti-BRG1 Antibody, clone 3G4 Certificates of Analysis

TitleLot Number
Anti-BRG1, clone 3G4 - 2207807 2207807
Anti-BRG1, clone 3G4 - 2291727 2291727
Anti-BRG1, clone 3G4 - 3925336 3925336
Anti-BRG1, clone 3G4 - 4052786 4052786
Anti-BRG1, clone 3G4 - 4094577 4094577
Anti-BRG1, clone 3G4 - NRG1782322 NRG1782322
Anti-BRG1, clone 3G4 -2688653 2688653
Anti-BRG1, clone 3G4 -2724251 2724251
Anti-BRG1, clone 3G4 Monoclonal Antibody 3098928
Anti-BRG1, clone 3G4 Monoclonal Antibody 3098927
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References

Reference overviewApplicationPub Med ID
Production of a rat monoclonal antibody against Brg1.
Ohkawa, Yasuyuki, et al.
Hybridoma (Larchmt), 28: 463-6 (2009)  2009

Show Abstract 		Immunocytochemistry20025509 20025509