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AB3403-I Anti-Beta Galactosidase Antibody, E. coli

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AB3403-I
100 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
BactWBChAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3403-I
ReplacesAB3403
DescriptionAnti-Beta Galactosidase Antibody, E. coli
Alternate Names
  • Beta-galactosidase
  • Acid beta-galactosidase
  • Lactase
  • Elastin receptor 1
Background Informationβ Galactosidase, also known as Beta-galactosidase, Beta-gal, LacZ, or Lactase, and encoded by the gene lacZ, is the enzyme in bacteria that hydrolyzes galactosides (sugars chains containing galactose) in to monosaccharides. β Galactosidase is typically an extracellularly produced enzyme that hydrolyzes the beta-glycosidic bond between galactose and another organic moiety. In E. coli, the β Galactosidase gene is present as part of the inducible lac operon system which is activated in the presence of lactose when glucose level is low. Today, bacterial beta galactosidase is used in molecular biology as a reporter marker to monitor gene expression, and because the enzyme has two functional domains, it forms the basis for α-complementation which is the methodology behind for the blue/white screening of recombinant clones. The presence or absence of an active β-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by β-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid.
References
Product Information
FormatAffinity Purified
PresentationPurified chicken polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Beta Galactosidase Antibody, E. coli is a highly specific chicken polyclonal antibody & that targets β-Galactosidase & has been tested in western blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenRecombinant protein corresponding to E. Coli Beta Galactosidase.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostChicken
Species Reactivity
  • Bacteria
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GLB1
  • ELNR1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~120 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in TOP10/PCU19 E. Coli lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Beta Galactosidase in 10 µg TOP10/PCU19 E. Coli lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB3403-I 04053252941399