Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABN1727 Anti-Beta-IV spectrin Antibody, clone N393/76

Anti-Beta-IV spectrin Antibody, clone N393/76, Cat. No. MABN1727, is a highly specific mouse monoclonal antibody that targets beta-IV spectrin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: SPTBN4, KIAA1642, SPTBN3 UniProt Number: Q9H254
View Products on Sigmaaldrich.com
MABN1727
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RIHC, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1727
DescriptionAnti-Beta-IV spectrin Antibody, clone N393/76
Alternate Names
  • Spectrin beta chain
  • non-erythrocytic 4
  • Spectrin
  • non-erythroid beta chain 3
Background InformationSpectrin beta chain, non-erythrocytic 4 (UniProt: Q9H254; also known as Beta-IV spectrin, Spectrin, non-erythroid beta chain 3) is encoded by the SPTBN4 (also known as KIAA1642, SPTBN3) gene (Gene ID: 57731) in human. Beta-IV Spectrin belongs to the spectrin family of proteins and is abundantly expressed in brain and pancreatic islets. It belongs to the spectrin family of proteins and contains two CH (calponin-homology) domains, one PH domain, and 18 spectrin repeats. It has been isolated as an interactor of the receptor tyrosine phosphatase ICA512. Spectrin molecule is assembled as a tetramer of two alpha and two beta subunits. A tight association between the amino-terminal domain of an alpha subunit and the carboxyl-terminal domain of a beta subunit forms a heterodimer. In brain, beta-IV Spectrin co-localizes with ankyrin-G 480/270-kDa at initial segments and nodes of Ranvier of myelinated neurons. In brain its highest expression is observed in the cell bodies of large myelinated neurons. It is not detected in non-neuronal cells, including glial and endothelial cells.Beta-IV Spectrin along with ankyrin-G is required for the anchoring of voltage-gated Na+ channels and for cell adhesion molecule anchoring to the actin cytoskeleton. (Ref.: Berghs, S et al. (2000). J. Cell Biol. 151(5): 985-1002).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Beta-IV spectrin Antibody, clone N393/76, Cat. No. MABN1727, is a highly specific mouse monoclonal antibody that targets beta-IV spectrin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected beta-IV spectrin in human pancreas, human cervical cancer, and human uterus tissue.

Western Blotting Analysis: A representative lot detected beta-IV spectrin in COS cells transiently transfected with Myc-tagged beta 4-spectrin.

Immunohistochemistry Analysis: A representative lot detected beta-IV spectrin in rat cerebral cortex and hippocampus tissues.
Biological Information
ImmunogenA recombinant fusion fragment corresponding to 192 amino acids from the C-terminal half of human beta-IV spectrin.
CloneN393/76
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone N393/76 specifically detects human and rat beta-IV Spectrin by immunohistochemistry. it targets a sequence within 192 amino acids in the C-terminal half of human beta-IV Spectrin.
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SPTBN4
  • KIAA1642
  • SPTBN3
Purification MethodProtein G purified
UniProt Number
Molecular Weight288.98 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human cerebellum tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected beta-IV spectrin in human cerebellum tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABN1727 04054839168246