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AB3738-I Anti-Beta Lactamase

Detect beta-lactamase using this rabbit Anti-Beta Lactamase polyclonal antibody, Cat. No. AB3738-I, validated for use in Western Blotting.

Anti-Beta Lactamase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: bla, blaT-3, blaT-4, blaT-5, blaT-6 UniProt Number: P62593
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AB3738-I
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3738-I
ReplacesAB3738
DescriptionAnti-Beta Lactamase
Alternate Names
  • Beta-lactamase TEM
  • IRT-4
  • Penicillinase
  • TEM-1
  • TEM-16/CAZ-7
  • TEM-2
  • TEM-24/CAZ-6
  • TEM-3
  • TEM-4
  • TEM-5
  • TEM-6
  • TEM-8/CAZ-2
Background InformationBeta-lactamase TEM (EC 3.5.2.6; UniProt P62593; also known as IRT-4, Penicillinase, TEM-1, TEM-16/CAZ-7, TEM-2, TEM-24/CAZ-6, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2) is encoded by the bla (also known as blaT-3, blaT-4, blaT-5, blaT-6) gene (Gene ID 3244915) in E. coli. TEM-1 belongs to the class-A beta-lactamase family. Beta-lactamases produced by bacteria provide resistance to beta-lactam antibiotics, such as penicillins and cephamycins by breaking open the beta-lactam ring. Over 200 beta-lactamases have been reported that are classified into four main groups (A, B, C, and D) and eight subgroups according to their functional and structural characteristics. The TEM-1 is predominant plasmid-mediated beta-lactamase of gram-negative rods. Six different nucleotide sequences, called blaTEM-1a, blaTEM-1b, blaTEM-1c, blaTEM-1d, blaTEM-1e, and blaTEM-1f, have been described to codify the same TEM-1 beta-lactamase. Ref.: Briñas, L., et al. (2002). Antimicrob. Agents Chemother. 46, 103156-103163.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect beta-lactamase using this rabbit Anti-Beta Lactamase polyclonal antibody, Cat. No. AB3738-I, validated for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged full-length E. coli beta-lactamase, including the signal peptide sequence.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects E. coli beta-lactamase.
Species Reactivity
  • All
Species Reactivity NoteE. Coli.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • bla
  • blaT-3
  • blaT-4
  • blaT-5
  • blaT-6
Purification MethodAffinity purified.
UniProt Number
Molecular Weight31.52/28.91 kDa (with/without signal peptide a.a. 1-23) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lasate from beta-lactamase-transformed E.coli.

Western Blotting Analysis: A 1:100,000 dilution of this antibody detected GST-tagged full-length E. coli beta-lactamase in 0.5 µg of lysate from transformed E. coli.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB3738-I 04054839112362