Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

07-1450-I Anti-Beta Pix, SH3 Domain Antibody

Detect Beta Pix using this Anti-Beta Pix antibody, SH3 Domain validated for use in western blotting.

Anti-Beta Pix, SH3 Domain Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: ARHGEF7, COOL1, P85SPR, PAK3BP, PIXB UniProt Number: Q14155
View Products on Sigmaaldrich.com
07-1450-I
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1450-I
Replaces07-1450
DescriptionAnti-Beta Pix, SH3 Domain Antibody
Alternate Names
  • Rho guanine nucleotide exchange factor 7
  • Beta-Pix
  • COOL-1
  • PAK-interacting exchange factor beta
  • p85
Background InformationBeta-Pix (ARHGEF7, βPix, COOL) is a Rho guanine nucleotide exchange factor (GEF). Beta-Pix is a p21-activated protein kinase (PAK)-interacting exchange factor (Pix, COOL, p85SPR) that has been identified as a putative guanine nucleotide exchange factor (GEF) for Rac/Cdc42. The variants of Beta-Pix isoforms exhibit different tissue distributions. Beta-Pix-a is expressed ubiquitously in all the tissues examined, and other isoforms are expressed mainly in the central nervous system. PAK, a Ser/Thr kinase, is an important mediator of Rho family activity interacts with Beta-Pix at the SH3 domain of Beta-Pix though its proline rich region. This interaction is thought to affect the GTP hydrolysis rate of Rac and Cdc42.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Beta Pix using this Anti-Beta Pix antibody, SH3 Domain validated for use in western blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected Beta-Pix, SH3 Domain in 10 µg of PC-12 cell lysate.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the SH3 domain of human Beta-Pix, SH3 Domain.
EpitopeSH3 domain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARHGEF7
  • COOL1
  • P85SPR
  • PAK3BP
  • PIXB
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~80 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Beta-Pix, SH3 Domain in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-1450-I 04053252958137

Documentation

Anti-Beta Pix, SH3 Domain Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Beta Pix, SH3 Domain Antibody Certificates of Analysis

TitleLot Number
Anti-Beta Pix, SH3 Domain - Q2388146 Q2388146
Anti-Beta Pix, SH3 Domain - 3267308 3267308
Anti-Beta Pix, SH3 Domain - 3425570 3425570
Anti-Beta Pix, SH3 Domain - 3597304 3597304
Anti-Beta Pix, SH3 Domain - 4070114 4070114
Anti-Beta Pix, SH3 Domain - 4134971 4134971
Anti-Beta Pix, SH3 Domain - 4198946 4198946
Anti-Beta Pix, SH3 Domain -2505110 2505110
Anti-Beta Pix, SH3 Domain -2570242 2570242
Anti-Beta Pix, SH3 Domain -2608851 2608851
Show more