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MABC1190 Anti-Bin1 Antibody, clone 2F11

Anti-Bin1, clone 2F11 Antibody, Cat. No. MABC1190, is a mouse monoclonal antibody that detects Bridging integrator 1 (Bin1) protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABC1190
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABC1190
DescriptionAnti-Bin1 Antibody, clone 2F11
Alternate Names
  • Myc box-dependent-interacting protein 1
  • Amphiphysin II
  • Amphiphysin-like protein
  • Box-dependent myc-interacting protein 1
  • Bridging integrator 1
Background InformationMyc box-dependent-interacting protein 1 (UniProt: O00499; also known as Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1) is encoded by the BIN1 (also known as AMPHL) gene (Gene ID: 274) in human. Bin1 is a ubiquitous protein that displays highest expression levels in the brain and muscle. Eleven isoforms of Bin1 have been described that are generated by alternate splicing. Isoform IIA is reported to be expressed only in the brain where it is concentrated n axon initial segments and nodes of Ranvier. It contains one BAR domain (aa 29-276) and one SH3 domain (aa 520-592). Bin1 interacts with the functionally critical Myc box regions at the N- terminus of the MYC oncoprotein and inhibits MYC-induced malignant cell transformation. Hence, it is considered as a tumor suppressor. Mutations in BIN1 gene are known to cause centronuclear myopathy that is characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Bin1, clone 2F11 Antibody, Cat. No. MABC1190, is a mouse monoclonal antibody that detects Bridging integrator 1 (Bin1) protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 4 µg/mL from a representative lot detected Bin1 in 10 µg of LNCaP and C2C12 cell lysates.

Immunohistochemistry Analysis: A representative lot detected Bin1 in Immunohistochemistry applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42).

Western Blotting Analysis: A representative lot detected Bin1 in Western Blotting applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42; Muller, A.J., et. al. (2003). Mol Cell Biol. 23(12):4295-306; Chang, M.Y., et. al. (2007). Cancer Res. 67(1):100-7).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to the BAR domain (aa 29-276) from the N-terminal half of human Myc box-dependent-interacting protein 1 (Bin1).
Epitopedomain of:
Clone2F11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2F11 recognizes an epitope within the N-terminal BAR domain of Bin 1.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
  • Chicken
  • Mouse
Species Reactivity NoteChicken, Human, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BIN1
  • AMPHL
Purification MethodProtein G purified
UniProt Number
Molecular Weight64.70 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human cerebellum and human skeletal muscle tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Bin1 in human cerebellum and human skeletal muscle tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC1190 04054839253119

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