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MABS2321-100UG Anti-Biotin Antibody, clone 8H6.8

Anti-Biotin, clone 8H6.8, Cat. No. MABS2321, is a mouse monoclonal antibody that detects Biotin-conjugated proteins and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

NewMABS2321-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABS2321-100UG
DescriptionAnti-Biotin Antibody, clone 8H6.8
Alternate Names
  • Vitamin B7
Background InformationBiotin, a bicyclic compound, is a water-soluble vitamin with a ureido (N-CO-N) and a tetrahydrothiophene ring. The ureido group is involved in its binding to avidin. The tetrahydrothiophene group is a valeric acid moiety. Although biotin molecule can exist in eight stereoisomers, the D-biotin isomer is the only one with biological activity. It plays important roles in a variety of critical metabolic reactions in the cell, and thus, is essential for normal human health, growth, and development. Its deficiency, particularly during pregnancy, leads to embryonic growth retardation, congenital malformation, and death. In mammals, biotin serves as a cofactor for four carboxylases that catalyze the transfer of a carboxyl group to targeted substrates. The four biotin-dependent carboxylases are: pyruvate carboxylase; propionly-CoA carboxylase; β-methylcrotonyl-CoA carboxylase; and acetyl-CoA carboxylase. These carboxylases play a critical role in the intermediate metabolism of gluconeogenesis, fatty acid synthesis, and amino acid catabolism. It is also reported to play a role in normal immune functions, including the production of antibodies, normal macrophage function, and the differentiation of T and B lymphocytes. This monoclonal antibody recognizes biotin conjugated to various proteins in ELISA and other immunochemical applications. (Ref.: Said, SM. (2012). Subcell. Biochem. 56; 1-19; Rabin, BS. (1983). J. Nutr. 113(11); 2316 2322).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Biotin, clone 8H6.8, Cat. No. MABS2321, is a mouse monoclonal antibody that detects Biotin-conjugated proteins and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
Key Applications
  • ELISA
  • Immunofluorescence
  • Western Blotting
Application NotesTested Applications

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Biotin-BSA.

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected Biotin-conjugates in Human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenBiotin conjugated to KLH.
EpitopeUnknown
Clone8H6.8
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 8H6.8 is a mouse monoclonal antibody that detects biotin-conjugated proteins.
IsotypeIgG1κ
Species Reactivity
  • All
Species Reactivity Note. Predicted to react with All based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Molecular Weight244.31 Da calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with Biotin-BSA.

ELISA Analysis: Serial dilutions from a representative lot detected Biotin-BSA in ELISA application.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS2321-100UG 04065271175020