Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

ABN68 Anti-Bystin Antibody

Anti-Bystin Antibody is an antibody against Bystin for use in WB, IH(P).

Anti-Bystin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Adhesion (CAMs) Gene Symbol: BYSL UniProt Number: Q13895
View Products on Sigmaaldrich.com
ABN68
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, Ca, Rhesus Macaque, Eq, R, BWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN68
DescriptionAnti-Bystin Antibody
Background InformationBystin and tastin serve as cytoplasmic proteins that are required for trophinin to mediate cell-to-cell adhesion between trophoblastic and endometrial epithelial cells in human placenta. Although the trophinin gene is unique to mammals, the bystin gene (BYSL) is conserved across eukaryotes. It is shown that bystin influences the transition from silent trophectoderm to an active trophoblast through trophinin-mediated cell adhesion. It is also essential for embryonic stem cell survival and trophectoderm development in mouse. Bystin is demonstrated to play a key role in ribosomal biogenesis by processing 18S RNA in the 40S subunit in human cancer cells and mouse embryos. It is also reported that c-MYC targets BYSL, which corresponds with bystin-mediated, rapid protein synthesis, and subsequent cellular growth.
References
Product Information
FormatAffinity Purified
Control
  • HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Bystin Antibody is an antibody against Bystin for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:300 dilution of this antibody detected Bystin in the syncytiotrophoblastic layer of the chorionic villi in human placenta.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Bystin.
EpitopeC-terminus.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the C-terminus of Bystin.
Species Reactivity
  • Human
  • Mouse
  • Canine
  • Rhesus Macaque
  • Horse
  • Rat
  • Bovine
Species Reactivity NoteDemonstrated to react with Human and Mouse. Predicted to react with Canine, Rhesus Macaque, Equine, Rat, and Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryBystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi.
Gene Symbol
  • BYSL
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. May be required for trophinin-dependent regulation of cell adhesion during implantation of human embryos.

SUBUNIT STRUCTURE: Binds trophinin, tastin and cytokeratins.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus > nucleolus. Note: Associated with 40S ribosomal subunits.
Molecular Weight~55 kDa observed. UniProtKB/Swiss-Prot Q13895 (BYST_HUMAN) states a molecular weight of ~49 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Bystin in 10 µg HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABN68 04053252592065